We narrowed to 18,153 results for: Met;

1. pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)

   Plasmid

   #173454

   Purpose

   Contains mScarletI-mNeonGreen tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mScarlet-I-mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKL09-GCaMP6f-mScarlet bb118

   Plasmid

   #158979

   Purpose

   Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   GCaMP6f-mScarlet fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#100833

   Promoter

   Bba_J23118 (sigma 70)

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCfB8788

   Plasmid

   #126922

   Purpose

   Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtI and HMG1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMXs-mRFP1

   Plasmid

   #21315

   Purpose

   MoMLV-based Retroviral vector, expresses mRFP1

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   monomeric Red Fluorescence Protein 1
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX459_gRNA-AAVS1_hspCas9

   Plasmid

   #193309

   Purpose

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)

   Plasmid

   #173455

   Purpose

   Contains mScarlet-mNeonGreen tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mScarlet-mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBAD-Citron1

   Plasmid

   #134300

   Purpose

   Bacterial expression of direct-response citrate sensor

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   Citron1
   

   Use

   Tags

   6-His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

   Plasmid

   #48295

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LZRS-IresGFP

   Plasmid

   #21961

   Depositor

   Lynda Chin

   Article

   Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

   Insert

   Ires GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   GACAGATGACTATGCAGAGAT

   Promoter

   Available since

   Sept. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

10. JunD

    Plasmid

    #187904

    Purpose

    Expresses JunD in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    JunD (Jund Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSF11-wemiRFP670-T2A-HO1

    Plasmid

    #197243

    Purpose

    Expresses the protein of wemiRFP670-T2A-HO1 in neurons of C elegan

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wemiRFP670-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-G-Kras

    Plasmid

    #180571

    Purpose

    ddGFP sensor of Kras

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddGFP sensor of Kras
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ER2-mhYFP

    Plasmid

    #186528

    Purpose

    Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHB-2

    Plasmid

    #140955

    Purpose

    For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R238S mutation in PhaC

    Promoter

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pICSL1P1_crU626

    Plasmid

    #191776

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CFP-FKBP

    Plasmid

    #162433

    Purpose

    for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

    Depositor

    Takanari Inoue

    Article

    Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

    Insert

    CFP-FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGWB701NL3F10H

    Plasmid

    #141288

    Purpose

    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Type

    Empty backbone

    Use

    Luciferase; Gateway

    Tags

    FLAG (x3), HIS (x10), and NLUC

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tUI-HA

    Plasmid

    #122662

    Purpose

    Express tUI-HA protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tUI-HA
    

    Use

    Tags

    HA-tag and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. C232-E05: attB2r-meGFP-attB3

    Plasmid

    #162902

    Purpose

    Gateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric enhanced Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENV8(WT)-GFPuv

    Plasmid

    #70127

    Purpose

    Cobalamin riboswitch reporter vector for use in E. coli

    Depositor

    Robert Batey

    Article

    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

    Insert

    wild type environmental cobalamin riboswitch sequence (env8)
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCfB8786

    Plasmid

    #126920

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SCP2-CMVenh-luc

    Plasmid

    #22230

    Depositor

    James Kadonaga

    Article

    Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

    Insert

    SCP2 (super core promoter 2)-CMVenh luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF6a-FKBP-CD28

    Plasmid

    #113401

    Purpose

    Expresses FKBP-CD28 in mammalian cell

    Depositor

    Min Zhuang

    Article

    Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

    Insert

    FKBP-CD28
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNIR-Fbactin

    Plasmid

    #184677

    Purpose

    NIR-Fb to actin

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fbactin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX-4T-3-SpSuc1

    Plasmid

    #162558

    Purpose

    Expresses GST-tagged S.pombe Suc1 in E.coli

    Depositor

    Iain Hagan

    Article

    Singh et al Methods Mol Biol. 2021;2329:123-142. doi

    Insert

    Schizosaccharomyces pombe Suc1 (suc1 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p458 VQR

    Plasmid

    #101727

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VQR

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTR47m4

    Plasmid

    #104068

    Purpose

    Luciferase Reporter for Formaldehyde Sensing

    Depositor

    David Liu

    Article

    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

    Inserts

    xluxAB
    frmR
    frm Promoter m4
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    23r and 25th nucleotides in sequence mutated from…

    Promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT2SK

    Plasmid

    #59383

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-kan
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHpgRNA13

    Plasmid

    #160127

    Purpose

    To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1

    Depositor

    Yongjin Zhou

    Article

    Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

    Insert

    PTEF1-HH-gRNA-ADE2- HDV-TAMO
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CAG-BDFP1.6

    Plasmid

    #197199

    Purpose

    Expresses the protein of BDFP1.6 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    BDFP1.6
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)

    Plasmid

    #48296

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    His6 and MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPD937 ZF37x12-Compact_EYFP

    Plasmid

    #138932

    Purpose

    EYFP reporter vector for the ZF37x12-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF37x12-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF37x12-Compact

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT2SC

    Plasmid

    #59382

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGC1::YC3.60

    Plasmid

    #107061

    Purpose

    Expressing Ca sensor yellow cameloen 3.6 under guard cell specific promoter

    Depositor

    Julian Schroeder

    Article

    Yang et al Plant Methods. 2008 Feb 19;4:6. doi: 10.

    Insert

    YC3.6
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    GC1 (At1g22690)

    Available since

    April 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SOX2se-CPG-TV

    Plasmid

    #70155

    Purpose

    a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
    

    Use

    Mouse Targeting and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPD548 ZF54x6-Compact_EYFP

    Plasmid

    #138862

    Purpose

    EYFP reporter vector for the ZF54x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF54x6-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF54x6-Compact

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-R-Hras

    Plasmid

    #180574

    Purpose

    ddRFP sensor of Hras

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddRFP sensor of Hras
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. miRFP718nano-based inflammation reporter

    Plasmid

    #195753

    Purpose

    Reporter of inflamation

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    NF-kB-RE-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPD296 ZF43x12-Compact_EYFP

    Plasmid

    #138907

    Purpose

    EYFP reporter vector for the ZF43x12-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x12-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x12-Compact

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDH-F-PA1

    Plasmid

    #40618

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

    Insert

    PA1 (PAGR1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    EF1

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only