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pEGFP-C3-PLK4-S285A/T289A-3xFLAG

Plasmid

#69841

Purpose

This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S285A/T289A

Promoter

CMV

Available Since

Dec. 3, 2015

Availability

Academic Institutions and Nonprofits only

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mTFP1-Caveolin-C-10

Plasmid

#55468

Purpose

Localization: Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

NM_001753 (CAV1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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p199 Dync1i2.C (Ex1a)

Plasmid

#26449

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
pTetOne-human cGAS-HA

Plasmid

#186896

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with C-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 WT

Plasmid

#184627

Purpose

Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 WT (KBTBD4 Human)

Expression

Mammalian

Mutation

no mutation

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1 KBTBD4 WT

Plasmid

#184624

Purpose

Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

no mutation

Promoter

TRE promoter, Tet ON

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-hPOT1_IRES-blast

Plasmid

#166411

Purpose

express hPOT1 in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 (POT1 Human)

Use

Lentiviral

Tags

myc

Expression

Mammalian

Promoter

ef-1a

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159858

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pG23A

Plasmid

#102884

Purpose

Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Pmyo3::tag:sl1(G16C)

Plasmid

#79003

Purpose

Expression of exogenous SL1 in C. elegans muscle

Depositor

Xiao Liu, Ge Zhan

Article

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Tags

illumina-adapter

Expression

Worm

Mutation

G16C

Promoter

myo-3

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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5HT2C-RNA1-FLAG

Plasmid

#79678

Purpose

serotonin receptor 2C RNA isoform 1 with C-terminal flag tag

Depositor

Stefan Stamm

Article

Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

Insert

5HT2C-RNA1 (HTR2C Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only
Human NCKX2-AS-long-FlagTag (pcDNA3.1+)

Plasmid

#75203

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C1C2-GFP

Plasmid

#46044

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV immediate early

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACt-mCherry-NLS

Plasmid

#138214

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NLS (Adcy10 Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACt-mCherry-NES

Plasmid

#138215

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NES (Adcy10 Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro

Plasmid

#154263

Purpose

Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE3G

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac SpyTag-β2AR-SpyTag

Plasmid

#135620

Purpose

Expression of SpyTag-B2AR in insect cell

Depositor

Hee-Jung Choi

Article

Choi et al Science. 2019 Nov 29;366(6469):1150-1156

Insert

Beta 2 adrenergic receptor (ADRB2 Human)

Tags

HA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tag

Expression

Insect

Mutation

Deleted 1-24 aa, 366-413 aa

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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MoFGFR3b-SV40PA

Plasmid

#86493

Purpose

mamalian cell expression of FGFR3b

Depositor

David Ornitz

Article

Chellaiah et al J Biol Chem. 1994 Apr 15;269(15):11620-7

Insert

FgfrR3 IIIb 12F5 (Fgfr3 Mouse)

Expression

Mammalian

Promoter

Moloney murine leukemia virus LTR

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2sh-HA

Plasmid

#97072

Purpose

Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

HA

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pdCas9-M-4A6

Plasmid

#73434

Purpose

CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Repressor 4A6 (orthogonal T7-lac repressor)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Constitutive wild-type S. pyogenes promoter

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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