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3538 pMIG Bad

Plasmid

#8787

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bad (Bad Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 19, 2005

Availability

Academic Institutions and Nonprofits only
pLmoCascade-IL1RNcrRNA

Plasmid

#126487

Purpose

Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Lmo IL1RN cr03

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSicoR-ADAM29i1

Plasmid

#59606

Purpose

Expression of shRNA against human ADAM29

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM29 (ADAM29 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-h-mmPtch1-FL-TagBFP

Plasmid

#120921

Purpose

Expresses BFP tagged Ptch1

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSI-535

Plasmid

#131130

Purpose

gRNA expression vector for C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

C-to-T reporter activation gRNA

Use

CRISPR

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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TDP43 NOTAG3

Plasmid

#28208

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Expression

Mammalian

Mutation

aa170-414 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pMA-Thermal_CTLA-4

Plasmid

#199751

Purpose

Thermal expression of CTLA-4

Depositor

Mikhail Shapiro

Article

Abedi et al Nat Commun. 2022 Mar 24;13(1):1585. doi:

Insert

CTLA-4 (Ctla4 Mouse)

Expression

Bacterial

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX2T merlin 1-332

Plasmid

#11631

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino-terminal half of merlin.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

Plasmid

#192406

Purpose

To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Turbo GFP

Use

Synthetic Biology

Tags

N7

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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ch-Tog

Plasmid

#28290

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

ch-TOG (CKAP5 Human)

Expression

Bacterial and Mammalian

Mutation

K19R, E118G

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only

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pTXB3 AurA

Plasmid

#68270

Purpose

bacterial expression of human AurA

Depositor

Stefano Ferrari

Article

Krystyniak et al Oncogene. 2006 Jan 19;25(3):338-48.

Insert

AurA (AURKA Human)

Tags

Mxe intein/chitin binding domain

Expression

Bacterial

Mutation

See depositor comment below

Promoter

T7

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pCB128

Plasmid

#205137

Purpose

Expression of mCherry-2xFYVE in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

2xFYVE (from human Hrs) (HGS Synthetic)

Use

Dictyostelium expression

Tags

mCherry

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pQZ083

Plasmid

#106417

Purpose

Express Complexin-1 fragment (1-83) in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Insert

complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)

Tags

GST tag

Expression

Bacterial

Mutation

Fragment 1-83, Codon optimization

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

Plasmid

#132682

Purpose

AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

AAV

Tags

6xHIS and Flag

Promoter

Chicken b-actin

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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GWF180

Plasmid

#133607

Purpose

CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

DNCR2-VPR, NS3aH1-tagBFP-SpdCas9

Expression

Mammalian

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro-{beta}4 SCR

Plasmid

#16267

Depositor

Arthur Mercurio

Article

Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

Insert

SCR Beta 4 Integrin (ITGB4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

Plasmid

#192380

Purpose

To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL704

Plasmid

#140374

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-4BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX2T merlin 340-595

Plasmid

#11634

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal half of merlin isoform 1.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pBI101.1/ProGOXL1:GUS

Plasmid

#175557

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL1

Tags

GUS

Expression

Plant

Promoter

GOXL1

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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