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pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

Plasmid

#185918

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Insert

human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pL302-HA-SUMO2

Plasmid

#235497

Purpose

Expression of HA-SUMO2

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

SUMO2 (SUMO2 Mouse, Human)

Use

Lentiviral

Tags

HA

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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p5078 pcDNA4c hBrd4 (1-470)

Plasmid

#14454

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1-470) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1-470

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
p5077 pcDNA4c hBrd4 (1-594)

Plasmid

#14453

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1-594) (BRD4 Human)

Tags

His

Expression

Mammalian

Mutation

amino acids 1-594

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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px330-UFL1 sgRNA2

Plasmid

#134636

Purpose

contains sgRNA targeting human UFL1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFL1 sgRNA2 (UFL1 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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px330-UFL1 sgRNA1

Plasmid

#134635

Purpose

contains sgRNA targeting human UFL1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFL1 sgRNA1 (UFL1 Human)

Expression

Mammalian

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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hNedd4-2 CS

Plasmid

#86041

Purpose

full length NeddL3 with C to S mutation with His Xpress tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

Insert

hNeddL3 with C to S mutation in HECT domain (NEDD4 Human)

Tags

His and Xpress

Expression

Mammalian

Mutation

C942S mutation in HECT domain

Promoter

CMV

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-His-myc-Uba1

Plasmid

#29508

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Uba1 (UBA1 Budding Yeast)

Tags

His and myc

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
GST-AR-4-52

Plasmid

#104199

Purpose

Expresses human AR-4-52 in in pGEX-4T-1 as GST fusion protein

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

human androgen receptor aa 4-52 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S81A-P82A

Plasmid

#171250

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82A)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Ala mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82…

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S81A-P82G

Plasmid

#171251

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82G)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Gly mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82…

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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GST-AR-1-36

Plasmid

#104192

Purpose

Expresses human AR aa 1-36 in pGEX-5x-1 bact expression vector with GST

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-36 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-αTAT1[2-236]

Plasmid

#27101

Depositor

Maxence Nachury

Article

Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

αTubulin K40 acetyltransferase (ATAT1 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pBG-ttLbUV2

Plasmid

#231386

Purpose

LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

Depositor

Qi-Jun Chen

Article

Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

Insert

ttLbCas12a Ultra V2

Use

CRISPR

Expression

Plant

Mutation

D156R and E795L

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR

Plasmid

#89078

Purpose

mammalian expression of human androgen receptor in pCMV5

Depositor

Elizabeth Wilson

Article

Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

Insert

human androgen receptor (hAR) full-length (AR Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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p4947 pcDNA4c hBrd4 full-length

Plasmid

#14441

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (BRD4 Human)

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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GST-AR-1-660

Plasmid

#104196

Purpose

Expresses human AR-1-660 from bacterial expression vector pGEX-5X-1 as GST fusion protein

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-660 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Sstr3-GFP-DEST

Plasmid

#49098

Purpose

Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Sstr3 (Sstr3 Mouse)

Tags

GFP and acceptor peptide (AP)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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Sc++

Plasmid

#155011

Purpose

Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

Insert

Sc++

Expression

Mammalian

Mutation

T1227K, Loop Substitution from S. anginosus

Promoter

EF1ɑ

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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AA184

Plasmid

#216047

Purpose

Fragmid fragment: (C' terminus) for prime editing; using PEmax

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

SV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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