We narrowed to 19,663 results for: Met
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Plasmid#64863PurposeExpresses a scrambled shRNADepositorInsertScrambled shRNA
UseRetroviralPromoteru6Available SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB655
Plasmid#60142PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationA4VPromoterMET17prAvailable SinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_ManII-SBP-mCherry
Plasmid#65259Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseLentiviralTagsStreptavidin Binding Protein (SBP) and mCherryMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-3xFLAG-LaG-2
Plasmid#172765PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)DepositorInsertLaG-16-3xFLAG-LaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDS240
Plasmid#34966PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationK533∆, high-affinity epitopePromoterGAL1Available SinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3G-mCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SUMO_LPETG_MT3
Plasmid#200304PurposeBacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavageDepositorAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9 fused to nanobody (anti-beta catenin)
Plasmid#186420PurposeExpression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tagDepositorInsertdCas9-nanobody fusion (anti-beta catenin)
UseCRISPRTags6HisAvailable SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mCardinal2
Plasmid#52631Purposebacterial expression vector with far-red FP mCardinal2DepositorInsertmCardinal2
ExpressionBacterialAvailable SinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
AF1521-c003
Plasmid#173111PurposeExpression of recombinant fusion protein in E. coliDepositorInsertAF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
TagsNanolucExpressionBacterialMutationKH-M1-L192Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-TGM2
Plasmid#69239PurposeU6 driven SpCas9 sgRNA expression for TGM2 siteDepositorAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxCerulean3
Plasmid#103975PurposePlasmid encoding moxCerulean3DepositorInsertmoxCerulean3
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMV-LEU2
Plasmid#65334Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUsDepositorInsertLEU2
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xMS2_SL]
Plasmid#68426PurposeTransient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing three MS2 Stem-loops
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
M-ST1n-VP64
Plasmid#48675PurposeMammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimizedDepositorInsertCas9-VP64, nuclease-null
UseCRISPRTagsNLS and VP64Mutationnuclease-null (D9A D598A H599A N622A)PromoterCMVAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM165/166-48nt
Plasmid#84091Purposetarget miRNA165/166 for destruction in Arabidopsis thalianaDepositorInsertSTTM165/166
ExpressionPlantPromoter2x35SAvailable SinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
anti-Notch2_B6-pBIOCAM5
Plasmid#39347DepositorInsertscFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)
Tags3xFLAG, 6xHis, and human FcExpressionMammalianAvailable SinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mCherry
Plasmid#175507PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3G-mCMVAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano140/pcDNA3
Plasmid#51966Purposemammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nMDepositorInsertYellow Cameleon-Nano140
ExpressionMammalianAvailable SinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-NtermN1
Plasmid#51444PurposeExpresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domainDepositorInsertTALEN CCR5 Left (L18) N1 N-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK150QPromoterCMVAvailable SinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only