We narrowed to 3,547 results for: pir
-
Plasmid#154845PurposeSynchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)DepositorInsertStx5 (Short isoform) (STX5 Human)
TagsStreptavidin Binding Protein (SBP) and mCitrineExpressionMammalianMutationLacks amino acids 1-54, enocdes short isoform of …PromoterCMVAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA LTB4R
Plasmid#226810PurposeTet-inducible lentiviral vector for 2xHA LTB4R expressionDepositorInsertLTB4R (LTB4R Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_858043.1Available SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G
Plasmid#204191PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralExpressionMammalianMutationDeletion of R162PromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G
Plasmid#204213PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralExpressionMammalianMutationPoint mutation on I207MPromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLbCpf1-BE-YE2
Plasmid#154146PurposeExpresses dLbCpf1-BE-YE2(dLbCas12a-BE-YE2) in mammalian cells.DepositorInsertdLbCpf1-BE-YE2 (dCas12a-BE-YE2)
TagsSV40 NLSExpressionMammalianMutationAPOBEC1(W90Y+R132E), dLbCpf1(D832A+E925A+D1148A)PromoterCMVAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Mouse-caspase-1/11a
Plasmid#183391PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Synthetic, Mouse)
UseRetroviralTagsMycPromoterMESVAvailable SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-redesigned-human-Caspase-4_ANR261ENP_D315H_M318K_T352K
Plasmid#183357PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4 with ICE activity (CASP4 Human)
UseRetroviralTagsMycMutationANR261ENP, D315H, M318K, T352KPromoterMESVAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+mouse-Caspase-11-catalytic domain
Plasmid#183396PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domains of canine caspase-1/4 fused to catalytic domain of mouse caspase-11
UseRetroviralTagsMycPromoterMESVAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+human-Caspase-4-catalytic domain
Plasmid#183395PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domains of canine caspase-1/4 fused to catalytic domain of human caspase-4
UseRetroviralTagsMycPromoterMESVAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S293A
Plasmid#115204PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2
Plasmid#115199PurposeLentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S291A
Plasmid#115203PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Flag-Myc-Qin-Tudor-attB
Plasmid#44210DepositorInsertQin-Tudor-domain (qin Fly)
UseFly transformationTagsFlag and MycMutationlacks E3 domain on N-term (del aa1-274)Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G
Plasmid#204214PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralExpressionMammalianMutationDeletion of DNA binding domain (delDBD)PromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G
Plasmid#204211PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralExpressionMammalianMutationPoint mutation on Q175* (*termination)PromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G
Plasmid#204210PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralExpressionMammalianMutationPoint mutation on R178* (*termination)PromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G
Plasmid#204212PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpressionDepositorInsertNK2 homeobox 1 (NKX2-1 Human)
UseLentiviralTagsPoint mutation on I207FExpressionMammalianPromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLbCpf1-BE-YEE
Plasmid#154148PurposeExpresses dLbCpf1-BE-YEE(dLbCas12a-BE-YEE) in mammalian cells.DepositorInsertdLbCpf1-BE-YEE (dCas12a-BE-YEE)
TagsSV40 NLSExpressionMammalianMutationAPOBEC1(W90Y+R126E+R132E), dLbCpf1(D832A+E925A+D1…PromoterCMVAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Hybrid-caspase_mouse-caspase-11-CARD+canine-Caspase-1/4-catalytic domain
Plasmid#183398PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domain of mouse caspase-11 fused to catalytic domain of canine caspase-1/4
UseRetroviralTagsMycPromoterMESVAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S291A/S293A
Plasmid#115205PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A/S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291A/S293A (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only