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pAc5.1B-lambdaN-HA-DmAGO1-D661K-siRNAres_J

Plasmid

#146714

Purpose

Insect Expression of DmAGO1-D661K-siRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-D661K-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K657E-siRNAres_J

Plasmid

#146715

Purpose

Insect Expression of DmAGO1-K657E-siRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K657E-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-Y653L-siRNAres_J

Plasmid

#146716

Purpose

Insect Expression of DmAGO1-Y653L-siRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-Y653L-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-CBS (H65R)

Plasmid

#182924

Purpose

Mammalian expression plasmid for human CBS mutant H65R

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

Cystathionine Beta-Synthase (CBS Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed histidine 65 to arginine

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED(216-242)+CBPTAZ1(340-439)

Plasmid

#189961

Purpose

Coexpression of CBP TAZ1 and CITED in E coli

Depositor

Peter Wright

Article

Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Inserts

CITED2 (216-242) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)

Plasmid

#189963

Purpose

Coexpression of CBP TAZ1 and CITED2 in E coli

Depositor

Peter Wright

Article

Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Inserts

CITED2 (216-269) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Expression

Bacterial

Mutation

L243A, E245A, L246A

Promoter

t7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CITED2(216-246)+CBP TAZ1(340-439)

Plasmid

#189962

Purpose

Expression of CBP TAZ1 and CITED2 in E coli

Depositor

Peter Wright

Article

Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Inserts

CITED2 (216-246) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Expression

Bacterial

Promoter

t7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRB121

Plasmid

#181945

Purpose

aTc-inducible expression of dimerized mNeonGreen (mNG-mNG)

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNeonGreen-mNeonGreen; tetR (AX04_RS23485 Synthetic)

Expression

Bacterial

Mutation

See Depositor Comments Below

Promoter

mNG-mNG-PLtetO-1; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K690A_M

Plasmid

#146957

Purpose

Insect Expression of DmAGO1-K690A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K690A (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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plIVMD217

Plasmid

#160939

Purpose

Excess Capsid Protein in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mTagBFP2-HA Tag

Tags

HA tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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plIVMD118

Plasmid

#160938

Purpose

Excess Capsid Protein in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mCherry-FLAG tag

Tags

MYC tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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plIVMD115

Plasmid

#160937

Purpose

Excess Capsid Protein for in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mCherry-MYC tag

Tags

MYC tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1160c2

Plasmid

#159724

Purpose

Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MPGIL-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1674

Plasmid

#159726

Purpose

Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MPTLV-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1479a3

Plasmid

#159725

Purpose

Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Tags

Gal4DBD and MYITV-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCSJ392

Plasmid

#159722

Purpose

Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

GID10 (GID10 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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GtCCR1_EYFP_pcDNA3.1

Plasmid

#159120

Purpose

This plasmid encodes GtCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

Insert

GtCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGAD-GH-Mefv iso1

Plasmid

#92107

Purpose

Yeast two hybrid

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-Metavinculin tail

Plasmid

#107152

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

Insert

metavinculin (VCL Human)

Tags

(His)8

Expression

Bacterial

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pETconNK-LGK

Plasmid

#81165

Purpose

pETconNK with levoglucosan kinase

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

Levoglucosan kinase wild-type

Tags

c-myc

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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