We narrowed to 5,886 results for: ARL

1. pAAV-EF1a-tdTomato-WPRE-pGHpA

   Plasmid

   #67527

   Purpose

   "Expresses the fluorescent protein tdTomato"

   Depositor

   Botond Roska

   Article

   Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

   Insert

   tandem dimer tomato
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOS003-lenti-CMV-GCaMPer

   Plasmid

   #65227

   Purpose

   ER calcium sensor for lentivirus production

   Depositor

   Adam Cohen, Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   GCaMPer
   

   Use

   Lentiviral

   Tags

   circularly permuted GFP

   Expression

   Mammalian

   Mutation

   GCaMP3 (D324G, D360G, D397G, D435G)

   Promoter

   CMV

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRVdGL-4Flpo

   Plasmid

   #98040

   Purpose

   Second-generation rabies viral vector genome

   Depositor

   Ian Wickersham

   Article

   Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

   Insert

   Flpo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_hSyn-fDIO-somQuasAr6a-EGFP-P2A-sombC1C2TG

   Plasmid

   #183537

   Purpose

   Voltage indicator

   Depositor

   Karl Deisseroth

   Article

   Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

   Insert

   somQuasAr6a-EGFP-P2A-sombC1C2TG
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   hSyn

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-Flpo

   Plasmid

   #55637

   Purpose

   Expresses Flpo in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV1, and AAV2

   Insert

   Flpo
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO ChRmine-eYFP-WPRE

   Plasmid

   #130996

   Purpose

   Double floxed ChRmine-eYFP under the control of Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   eYFP

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-MDFI

   Plasmid

   #207836

   Purpose

   Tagged MDFI for western blotting and imaging

   Depositor

   Charles Cox

   Article

   Zhou et al Science. 2023 Aug 18;381(6659):799-804.

   Insert

   MDFI (MDFI Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pECE HA AMPKa1 WT

   Plasmid

   #69504

   Purpose

   Expresses AMPK alpha1 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Tag2B EDD

   Plasmid

   #37188

   Depositor

   Darren Saunders, Charles Watts

   Article

   Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

   Insert

   EDD (UBR5 Human)
   

   Use

   Tags

   6x His Tag and FLAG

   Expression

   Mammalian

   Mutation

   Please see Depositor Comments

   Promoter

   CMV

   Available since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-vCre

    Plasmid

    #55638

    Purpose

    EF1a-driven expression of VCre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Insert

    vCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

    Plasmid

    #154826

    Purpose

    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    mCherry
    NeoR
    ZsGreen1-DR
    

    Use

    CRISPR and Cre/Lox

    Tags

    PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, EF1a, and SV40 early promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. cyt-GafD-mTurboID-V5

    Plasmid

    #184640

    Purpose

    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    cyt-GlycoID-V5
    

    Use

    Tags

    Nuclear export sequence and V5 tag

    Expression

    Mammalian

    Mutation

    GafD -> expressed aa22-178 ; BirA* -> expre…

    Promoter

    CMV

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKRG3-mU6-PUb-hSpCas9-pAc

    Plasmid

    #162163

    Purpose

    Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    T2A-pAc

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MICU-APEX2

    Plasmid

    #79057

    Purpose

    A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    C-terminal APEX2 fused MICU1 (MICU1 Human, Soybean)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV immearly promotor

    Available since

    Sept. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. nuc-GafD-mTurboID-HA

    Plasmid

    #184641

    Purpose

    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    nuc-GlycoID-HA
    

    Use

    Tags

    3xHA tag and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    GafD -> expressed 22-178 ; BirA* -> express…

    Promoter

    CMV

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

    Plasmid

    #35510

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKIIa-eArch 3.0-EYFP

    Plasmid

    #35516

    Purpose

    AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    Arch3.0-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUW-tetO-lox-hKLF4

    Plasmid

    #20727

    Depositor

    Rudolf Jaenisch

    Article

    Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CaMKIIa-ChRmine-eYFP-WPRE

    Plasmid

    #130988

    Purpose

    AAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EF1a-ArgiNLS-mGreenLantern

    Plasmid

    #220596

    Purpose

    Constitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

    Plasmid

    #35501

    Purpose

    AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pECE M2-SH3PXD2A WT

    Plasmid

    #69813

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

    Plasmid

    #35512

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-Tag2B EDD C2768A

    Plasmid

    #37189

    Depositor

    Darren Saunders, Charles Watts

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD C2768A (UBR5 Human)
    

    Use

    Tags

    6x His Tag and FLAG

    Expression

    Mammalian

    Mutation

    Changed Cysteine 2768 to Alanine

    Promoter

    CMV

    Available since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

    Plasmid

    #162164

    Purpose

    Selectable homology donor template for Aag2 cell AGO1

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    RFP
    3xFLAG
    

    Use

    CRISPR and Cre/Lox; Homology donor template

    Tags

    AGO1 homology and loxP

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and endogenous AGO1

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5-FRT/TO-N-mEGFP-PARP11

    Plasmid

    #177998

    Purpose

    Expresses N-terminally mEGFP-tagged PARP11

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP11 (PARP11 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FUW-tetO-lox-hOCT4

    Plasmid

    #20728

    Depositor

    Rudolf Jaenisch

    Article

    Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-EF1a-CVS-G-WPRE-pGHpA

    Plasmid

    #67528

    Purpose

    "Expresses the rabies glycoprotein"

    Depositor

    Botond Roska

    Article

    Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

    Insert

    Rabies virus strain CVS-11 glycoprotein (G)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSyn-ChRmine-eYFP-WPRE

    Plasmid

    #130992

    Purpose

    AAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge