We narrowed to 13,611 results for: Mpl;

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pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS

Plasmid

#124652

Purpose

Chimeric ETS domain

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 βarr1 S412A Flag

Plasmid

#42195

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412A (Arrb1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

S412A

Promoter

CMV

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(84Q)

Plasmid

#21754

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 84 Glutamines and no…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pJZC25

Plasmid

#62328

Purpose

sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x MS2 binding module
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp5

Plasmid

#157686

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp5 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA4.TO-ORF61-2xCSTREP

Plasmid

#136221

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF61

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF61

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-del-N

Plasmid

#61004

Purpose

Expresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 delete N (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

deleted amino acids 17-310

Promoter

CMV

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-FH-Nsp2

Plasmid

#157683

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp2 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-GLI1

Plasmid

#79497

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

GLI1 (GLI1 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (208-439)

Plasmid

#203433

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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DNAJC11-EGFP

Plasmid

#210368

Purpose

Expresses DNAJC11 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

DNAJC11 (DNAJC11 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Orf6

Plasmid

#157727

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Orf6 (ORF6 SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA4.TO-K1-2xCSTREP

Plasmid

#136148

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K1

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCbs Flag CMYC (bHLH/LZ)

Plasmid

#203424

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-del-C

Plasmid

#61005

Purpose

Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 delete C (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

deleted amino acids 934-1187

Promoter

CMV

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA Myc-mMEKK2

Plasmid

#44158

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

Mekk2 (Map3k2 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
NSP14-Flag

Plasmid

#210346

Purpose

Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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bArr1-ALFA

Plasmid

#201496

Purpose

Expression of the beta-arrestin1 containing a C-terminal ALFA tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr1-ALFA (ARRB1 Human)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF33-2xCSTREP

Plasmid

#136194

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF33

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF33

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF60-2xCSTREP

Plasmid

#136220

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF60

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF60

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF38-2xCSTREP

Plasmid

#136199

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF38

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF38

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP

Plasmid

#90012

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C
NBirA*-GFP

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGnptg

Plasmid

#78106

Purpose

Mammalian expression of mouse Gnptg with C-terminal His tag

Depositor

Thomas Braulke

Article

Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

Insert

Gnptg (Gnptg Mouse)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE

Plasmid

#127868

Purpose

pAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1 (Nos1 Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Amino acids 1-133 only

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF63-2xCSTREP

Plasmid

#136223

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF63

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF63

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 Myc-huMEKK3 (K391A)

Plasmid

#44157

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

MEKK3 (K391A) (MAP3K3 Human)

Tags

Myc

Expression

Mammalian

Mutation

K391A, kinase-dead mutant

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-K8a-2xCSTREP

Plasmid

#136158

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8a

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K8a

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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Human FLAG-WDR20

Plasmid

#217618

Purpose

Mammalian expression of FLAG tagged WDR20

Depositor

Ethan Lee

Article

Ng et al Nat Commun. 2023 Oct 5;14(1):6173. doi:

Insert

WDR20 (WDR20 Human)

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJZC32

Plasmid

#62327

Purpose

sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-SynI-CreOn-FlpOff-MCS-P2A-EGFP

Plasmid

#176276

Purpose

Viral vector for co-expression of a CDS of interest and EGFP in cells expressing Cre AND NOT Flp driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

MCS-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Nsp8-HF

Plasmid

#157696

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-FRT-TO-Nsp7

Plasmid

#157688

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEBTet-SNAP-Cep57

Plasmid

#136817

Purpose

Mammalian expression of the centrosomal protein Cep57 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep57 (CEP57 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mNeon-Blast-TUBB4B

Plasmid

#207786

Purpose

Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST27-GST-ATXN1 FL(30Q)

Plasmid

#21753

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 32 amino acids (30 Q…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-ORF75-2xCSTREP

Plasmid

#136234

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF75

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF75

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
gp64-mVEGF-C-HisTag-pENTR

Plasmid

#64065

Purpose

Plasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.

Depositor

Joanna Bereta

Article

Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

Insert

vascular endothelial growth factor C (Vegfc Mouse)

Tags

His-tag

Expression

Insect

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LN-entry+NLS

Plasmid

#128011

Purpose

Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

LN-3xFlag-SV40NLS

Use

Lamdan entry vector with nls to generate tetherin…

Tags

LN-3xFlag-SV40NLS

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-Atg14 S29D

Plasmid

#86757

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

S29D

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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