We narrowed to 5,142 results for: mos

1. Frt-hspB1

   Plasmid

   #63092

   Purpose

   Expression of HSP1 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb1 (HSPB1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Polymerase expression - pCMV-ePhi29 (LM2802)

   Plasmid

   #208967

   Purpose

   Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. NC15 pGLUE CUL3

   Plasmid

   #36970

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   CUL3 (CUL3 Human)
   

   Tags

   CBP tag, HA, and streptag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

4. H14-MBP-SUMO-Cys-Linker-BAF_G47E

   Plasmid

   #101779

   Purpose

   Expresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Tags

   His14-MBP-SUMO

   Expression

   Bacterial

   Mutation

   G47E mutation

   Promoter

   T5

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

   Plasmid

   #208969

   Purpose

   Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   EcKlenow-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-eYFP-FKBP-MAD1-AA

   Plasmid

   #114038

   Purpose

   Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

   Depositor

   Geert Kops

   Article

   Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

   Insert

   Human MAD1 (MAD1L1 Human)
   

   Tags

   eYFP-FKBP12

   Expression

   Mammalian

   Mutation

   MAD2 binding deficient MAD1 K541A/L543A

   Promoter

   CMV

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-eDHFR-Cdc42Q61L

   Plasmid

   #107267

   Purpose

   mCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   mCherry-eDHFR-Cdc42Q61L (CDC42, dhfr Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG416GAL-ccdB-mRuby3

   Plasmid

   #166139

   Purpose

   Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Tags

   mRuby3

   Expression

   Yeast

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Frt-hspB8

   Plasmid

   #63099

   Purpose

   Expression of HSPB8 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb8 (HSPB8 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. KCNE2_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176980

    Purpose

    Mammalian expression vector encoding KCNE2 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    KCNE2 (KCNE2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOD2087-trnDEG

    Plasmid

    #89369

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Promoter

    Pmec-18

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2

    Plasmid

    #190898

    Purpose

    Entry vector for multiple sgRNA cloning

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6 and 7sk

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-shGBP1.1.mKO2

    Plasmid

    #85209

    Purpose

    TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GBP1 (guanylate binding protein 1) (GBP1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-tetpA-hNEUROG2-iRPT

    Plasmid

    #140764

    Purpose

    Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

    Inserts

    iRFP713
    PuroR
    rtTAM2
    NEUROG2 (NEUROG2 Human)
    

    Expression

    Mammalian

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

    Plasmid

    #108484

    Purpose

    inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    CenpB-dCEN-INCENP (INCENP Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    CEN box replaced by CenpB 1-166

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p4949 pLPCX-His-Xpress NLS hBrd4 CTD

    Plasmid

    #14459

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 CTD (BRD4 Human)
    

    Use

    Retroviral

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    amino acids 1047-1362 of Brd4 (the CTD). Function…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

17. Frt-V5-hspB2

    Plasmid

    #63103

    Purpose

    Expression of HSPB2 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb2 (HSPB2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV4Neo-murine IL-17RASEFIR-HA

    Plasmid

    #46863

    Purpose

    expresses mouse IL-17RA with SEFIR deletion

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA-delta-SEFIR (Il17ra Mouse)
    

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Deletion of SEFIR domain (AA 394-485)

    Promoter

    CMV

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

19. HMGN1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176943

    Purpose

    Mammalian expression vector encoding HMGN1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    HMGN1 (HMGN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. GUT BuGZ delta ZF2

    Plasmid

    #84026

    Purpose

    Expression of GFP tagged mutant BuGZ in human cells

    Depositor

    Patrick Paddison

    Article

    Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

    Insert

    BuGZ (ZNF207 Human)
    

    Use

    Lentiviral

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    delta 35-58; Q394R

    Promoter

    UCOE / SFFV

    Available Since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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