We narrowed to 30,971 results for: emb
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Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-H3.3-polyA
Plasmid#59780PurposeExpress Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorAvailable SinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
3XFlag pCMV5-TOPO TAZ WT
Plasmid#24809DepositorAvailable SinceMay 14, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
FB027
Plasmid#119712PurposeMultipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMTDepositorInsertPgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
UseSynthetic BiologyAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-CHMP2A
Plasmid#115330Purposeexpresses mCherry-CHMP2A in mammalian cellsDepositorAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
C1-mCitrine-FKBP-EZRabd(t567D)
Plasmid#155227PurposeAcute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with RapamycinDepositorInsertmCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
TagsmCitrineExpressionMammalianMutationChanged Thr 567 to Asp in original EzrinAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-KR-Linker-eGFP
Plasmid#176066PurposeEGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassetteDepositorInsertKR
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-NLS-EGFP-deadBtgh-3NLS
Plasmid#194470PurposeEncodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.DepositorInsertBtGH84
UseFor in vitro transcription from t3 promoter after…TagsSV40 Nuclear Localization Signal, SV40 Nuclear Lo…MutationAspartic Acid 242 (in Dennis et al.) changed to A…Available SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bin1 delta N-BAR
Plasmid#168282PurposeExpress N-BAR-deleted Bin1 mutant (aa 268-476)DepositorAvailable SinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-Delta23C11orf83
Plasmid#65849Purposebacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)DepositorInsertUQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
TagsGSTExpressionBacterialMutationdeletion of the 23 first aa (TM)Available SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only