We narrowed to 4,276 results for: 424

1. pDONR223_ILKAP_WT

   Plasmid

   #81809

   Purpose

   Gateway Donor vector containing ILKAP , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   ILKAP (ILKAP Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXS-IRES-BLAST flag-CAD hCPS1 AD

   Plasmid

   #188130

   Purpose

   Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pD649-HAsp-ICOS-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156498

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   ICOS (ICOS Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-sgACSL3.C-Cas9-P2A-Puro

   Plasmid

   #129412

   Purpose

   Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   SpCas9 and sgACSL3.C (ACSL3 Human)
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q52

   Plasmid

   #111748

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q52 (polyQ repeat)

   Promoter

   CMV and P10

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_NRAS_p.G12A

   Plasmid

   #81664

   Purpose

   Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NM_002524.4 (NRAS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   G12A

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q24

   Plasmid

   #111742

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q24 (polyQ repeat)

   Promoter

   CMV and P10

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-Basic-ACSL3-sfGFP(C) HDR template

   Plasmid

   #129413

   Purpose

   HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   ACSL3 HDR template (ACSL3 Human)
   

   Use

   CRISPR and TALEN; Endogenous tagging hdr template

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_DVL3_WT

   Plasmid

   #82267

   Purpose

   Gateway Donor vector containing DVL3, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DVL3 (DVL3 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   W433L

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti/TO/EYFP-P2A-IDH1(HA)

    Plasmid

    #122482

    Purpose

    A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and IDH1 C-terminally tagged with HA

    Depositor

    Eric Huang

    Article

    Tiburcio et al Acta Neuropathol. 2018 Feb;135(2):285-29

    Insert

    isocitrate dehydrogenase 1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    HA and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-DNER-COMP5AP-AviTag-9xHis

    Plasmid

    #157490

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    DNER (DNER Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXS-IRES-BLAST flag-CAD E1368A

    Plasmid

    #188141

    Purpose

    Expresses C-terminal flag-tagged human CAD E1368A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ATX3-77Q-R388G

    Plasmid

    #184251

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Human, Synthetic)
    

    Use

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    C-terminal codon optimization for protein expres…

    Promoter

    T7 promoter

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HisGB1-CITED2(216-269)+CBPTAZ1(340-439)

    Plasmid

    #173761

    Purpose

    Bacterial coexpression of human CITED2 CTAD with CBP TAZ1

    Depositor

    Peter Wright

    Article

    Berlow et al Nature. 2017 Mar 16;543(7645):447-451. d

    Inserts

    CITED2 (216-269) (CITED2 Human)
    CBP TAZ1 (340-439) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXS-IRES-BLAST flag-CAD S1396A

    Plasmid

    #188126

    Purpose

    Expresses C-terminal flag-tagged human CAD S1396A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR223_NRAS_WT

    Plasmid

    #82150

    Purpose

    Gateway Donor vector containing NRAS , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NES-EGFP-PH-BTKx1

    Plasmid

    #183651

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Human, Frog)
    

    Use

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 2-170

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTR-341 pUC19-tNGFR-P2A-PDCD1 N

    Plasmid

    #186074

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    PDCD1 (PDCD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-IDH1-P33S

    Plasmid

    #116409

    Purpose

    Lentiviral expression of IDH1 P33S

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    P33S

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-myc-AP1S3 F4C

    Plasmid

    #58293

    Purpose

    mammalian expression of AP1S3 F4C mutant

    Depositor

    Francesca Capon

    Article

    Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

    Insert

    AP1S3 (AP1S3 Human)
    

    Use

    Tags

    6xHis and myc

    Expression

    Mammalian

    Mutation

    F4C: c.11T>G, p.Phe4Cys

    Promoter

    CMV

    Available since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only