We narrowed to 6,241 results for: nls

1. pQP-T2A

   Plasmid

   #102583

   Purpose

   Light-controlled activation of expression

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

   Insert

   BphP1-VP16-T2A-NLS-GAL4-QPAS1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pG14-MS2-GFP

   Plasmid

   #27117

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2-binding protein
   

   Tags

   GFP, HA, and NLS

   Expression

   Yeast

   Available Since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBFv-nosP-Cas9

   Plasmid

   #138402

   Purpose

   Expresses Cas9 in the germline of Drosophila

   Depositor

   Shu Kondo

   Article

   Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

   Insert

   Cas9-NLS
   

   Expression

   Insect

   Promoter

   UAS

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRP2370

   Plasmid

   #127576

   Purpose

   Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
   

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Yeast

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRP2365

   Plasmid

   #127575

   Purpose

   Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection marker

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
   

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Yeast

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3s-Cas9HC

   Plasmid

   #43945

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA epitope and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRP2371

   Plasmid

   #127577

   Purpose

   Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection marker

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
   

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Yeast

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNXRVa-HACre

   Plasmid

   #78398

   Purpose

   constitutive Cre expression vector

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Inserts

   3xHA-nls-Cre
   kanMX6
   

   Expression

   Yeast

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. FRP2372

   Plasmid

   #127578

   Purpose

   Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection marker

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
   

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Yeast

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRP2374

    Plasmid

    #127579

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Expression

    Yeast

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVITRO1-SS-247

    Plasmid

    #68761

    Purpose

    ZF9-TF-V2 with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    ZF9-TF-V2
    mCherry
    

    Tags

    NLS and flag

    Expression

    Mammalian

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-ACME

    Plasmid

    #186717

    Purpose

    ACME dual-deaminase CRISPR base editor

    Depositor

    Xue Gao

    Article

    Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

    Insert

    ACME
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS406-CMV-lacI-3FLAG

    Plasmid

    #83410

    Purpose

    Expression of lacI in yeast

    Depositor

    Toshi Tsukiyama

    Article

    Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

    Insert

    lacI repressor
    

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Promoter

    CMV

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pISV-CRISPR/Cas9PGmUbi

    Plasmid

    #209189

    Purpose

    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection

    Depositor

    Christian Staehelin

    Inserts

    Cas9
    DsRed(deltaB)
    

    Tags

    NLS

    Expression

    Plant

    Mutation

    missing BsaI site and plant-codon optimized with …

    Promoter

    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMVJ-scTALEN-ABL1-R

    Plasmid

    #61695

    Purpose

    Expresses a single-chain TALEN targeting ABL1 site in human cells

    Depositor

    Huimin Zhao

    Article

    Sun et al Mol Biosyst. 2014 Mar 4;10(3):446-53. do

    Insert

    scTALEN-ABL1-R
    

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Available Since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pME-24xMBS

    Plasmid

    #86246

    Purpose

    Middle entry vector for generating MBS tagged RNAs

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    NLS-tdMCP

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIK86

    Plasmid

    #47933

    Purpose

    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.

    Depositor

    Iskra Katic

    Article

    Katic et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR and Unspecified

    Promoter

    T7

    Available Since

    Oct. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRVV655

    Plasmid

    #119019

    Purpose

    Drives TP901-1 recombinase expression in the D. melanogaster germ line

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

    Insert

    TP901-1 recombinase
    

    Tags

    SV40 NLS

    Expression

    Insect

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVITRO1-SS-113

    Plasmid

    #68737

    Purpose

    ZF9-TF with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    ZF9-VP64
    mCherry
    

    Tags

    NLS and flag

    Expression

    Mammalian

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVITRO1-SS-148

    Plasmid

    #68749

    Purpose

    ZF8/ZF7-TF with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    ZF8/ZF7-VP64
    mCherry
    

    Tags

    NLS and flag

    Expression

    Mammalian

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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