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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,730 results for: GEM;

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Showing: 511 - 540 of 2730 results

  1. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid
    #92139
    Purpose
    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92139-plasmid-map-sequence-id-349059

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  2. TRE-pfv-Sapphire

    Plasmid
    #231957
    Purpose
    The plasmid containing TRE-pfv-Sapphire for the doxycycline-inducible expression of genetically encoded multimeric nanoparticles (GEM)
    Depositor
    Michael Shtutman
    Article
    Korunova et al bioRxiv [Preprint]. 2024 Nov 18:2024.11.
    Insert
    genetically encoded multimeric nanoparticles
    Use
    Lentiviral; Doxocycline inducible
    Expression
    Mammalian
    Promoter
    tight TRE promoter
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231957-plasmid-map-sequence-id-462153

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  3. pAN1232

    Plasmid
    #127164
    Purpose
    Vector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)
    Depositor
    Hana El-Samad
    Article
    Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    pRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
    Use
    Synthetic Biology
    Available Since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    127164-plasmid-map-sequence-id-250325

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  4. pUBC-Pfv-Sapphire

    Plasmid
    #203649
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203649-plasmid-map-sequence-id-402479

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  5. TetCMV-Pfv-Sapphire

    Plasmid
    #203650
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the doxycycline-inducible promoter.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203650-plasmid-map-sequence-id-402480

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  6. pUBC-Pfv-Sapphire_PURO

    Plasmid
    #203651
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203651-plasmid-map-sequence-id-402481

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  10. pUBC-SV40NLS-PopZ3H-QtE-TSapphire

    Plasmid
    #250610
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 50nm nucQtE-GEMs under the UBC promoter
    Depositor
    Liam Holt
    Insert
    Encapsulin
    Use
    Lentiviral
    Tags
    PopZTag and TSapphire
    Expression
    Bacterial and Mammalian
    Mutation
    SV40NLS signal on N-terminal
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250610-plasmid-map-sequence-id-498552

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  11. pUBC-Pfv-Sapphire-IRES-H2B-mCherry

    Plasmid
    #203648
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire-IRES-H2B-mCherry
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203648-plasmid-map-sequence-id-402478

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  13. GMIP

    Plasmid
    #32046
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GMIP (GMIP Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 80-357 only
    Available Since
    Jan. 13, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pUBC-popTag-QtE-mScarlet

    Plasmid
    #240101
    Purpose
    Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.
    Depositor
    Liam Holt
    Article
    Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e
    Insert
    Encapsulin
    Use
    Lentiviral
    Tags
    mScarlet and popZ
    Expression
    Bacterial and Mammalian
    Promoter
    UBC promoter
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    240101-plasmid-map-sequence-id-476613

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  27. pHES839

    Plasmid
    #87941
    Purpose
    Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    GAL4 DBD - hER LBD - MSN2 AD (ESR1 Budding Yeast, Synthetic, Human)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    ADH1 (crippled)
    Available Since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87941-plasmid-map-sequence-id-180360

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Showing: 511 - 540 of 2730 results