We narrowed to 13,831 results for: Cas9

1. pH-nCas9-PPE-V2

   Plasmid

   #170131

   Purpose

   For plant prime editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

   Insert

   nCas9(H840A)-M-MLV
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   H840A for Cas9; D200N, T306K, W313F, T330P and L…

   Promoter

   maize Ubiquitin-1, OsU3

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP-dCas9-VPR

   Plasmid

   #63798

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9 TadCBEd

   Plasmid

   #193835

   Purpose

   Express TadCBEd (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDd-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. BB3cH_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104907

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-Geminin

   Plasmid

   #109401

   Purpose

   Expresses Cas9 fusion to Geminin degron domain

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   Geminin (GMNN Human)
   

   Use

   Tags

   Expression

   Mutation

   Geminin degron fragment

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9-hHDAC3

   Plasmid

   #98591

   Purpose

   3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

   Depositor

   Zhaolan Zhou

   Article

   Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

   Insert

   dCAS9-HDAC3 (HDAC3 Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1A

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCas9-EGFP

   Plasmid

   #63592

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Phil Sharp, Feng Zhang

   Article

   Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

   Inserts

   Cas9
   EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_Cas9–superNLS

   Plasmid

   #232430

   Purpose

   Expression plasmid for the SpCas9 nuclease with superNLS

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   Cas9–superNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-VPR_P2A_mCherry

   Plasmid

   #154193

   Purpose

   Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sp-dCas9-VPR_P2A_mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1as

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9_T2A_iRFP670 sgAAVS1

    Plasmid

    #192477

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459V2.0-HypaCas9

    Plasmid

    #108294

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-CjCas9

    Plasmid

    #89754

    Purpose

    Expression of CjCas9 with His tag in E.coli

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    6XHis, HA, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYLCRISPR/Cas9Pubi-B

    Plasmid

    #66188

    Purpose

    expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    maize ubiquitin promoter (Pubi)

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9_T2A_mCherry sgAAVS1

    Plasmid

    #192480

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pdCas9-Tet1-CD

    Plasmid

    #83340

    Purpose

    To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

    Depositor

    Ronggui Hu

    Article

    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

    Insert

    dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    U6, CBH

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCas9-KRAB

    Plasmid

    #110820

    Purpose

    Conventional dCas9 repressor-dCas9-KRAB

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-dCas9

    Plasmid

    #47106

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330A_dCas9-1x4

    Plasmid

    #63598

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9- AsiA_m2.1

    Plasmid

    #158065

    Purpose

    Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

    Depositor

    Harris Wang

    Article

    Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

    Insert

    dCas9-AsiA_m2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9-CtIP

    Plasmid

    #109403

    Purpose

    Expresses Cas9 fusion to human CtIP protein

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    CtIP (RBBP8 Human)
    

    Use

    Tags

    Expression

    Mutation

    full length CtIP

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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