Addgene

We narrowed to 11,394 results for: Lif;

Showing: 341 - 360 of 11394 results

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AdEasier-1 cells (strain)

Bacterial Strain

#16399

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Ampicillin and streptomycin

Species

Escherichia coli

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
bSLS.114

Bacterial Strain

#191530

Purpose

Contains a knockout gene in the E. coli strain BL21-AI

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Species

Escherichia coli

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only
bMS.346

Bacterial Strain

#220588

Purpose

Increase editing efficiency of retron and oligo recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only
pDYH5

Plasmid

#52032

Purpose

Expression of GFP-tagged DAF-16 in C. elegans

Depositor

Meng-Qiu Dong

Article

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

DAF-16 (daf-16 Nematode)

Use

Tags

GFP and HIS

Expression

Worm

Mutation

Promoter

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pTL-4

Plasmid

#52033

Purpose

Inducible expression of GST-unc43 in E. coli

Depositor

Meng-Qiu Dong

Article

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

Unc-43 (unc-43 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
scAAV-TTR-mFgf15

Plasmid

#190594

Purpose

AAV construct containing Fgf15 cds under control of TTR promoter

Depositor

Roel Nusse

Article

Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

Insert

Fgf15 (Fgf15 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ttr

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDYH5

Plasmid

#52032

Purpose

Expression of GFP-tagged DAF-16 in C. elegans

Depositor

Meng-Qiu Dong

Article

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

DAF-16 (daf-16 Nematode)

Use

Tags

GFP and HIS

Expression

Worm

Mutation

Promoter

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pTL-4

Plasmid

#52033

Purpose

Inducible expression of GST-unc43 in E. coli

Depositor

Meng-Qiu Dong

Article

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

Unc-43 (unc-43 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pNP151

Plasmid

#106359

Purpose

To insert a single copy of a construct at a site on Chr II, corresponding to a mKate-labelled LifeAct expressed specifically in the adult epidermis.

Depositor

Jonathan Ewbank

Article

Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

Insert

col-62p_lifeact_mkate2_3'nmy-2

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGI3EM22C

Plasmid

#135488

Purpose

Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr LifeAct-tdTomato

Use

Tags

LifeAct

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pmCitrine_N1

Plasmid

#92420

Purpose

Empty backbone for C-terminal fusion of mCitrine

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

mCitrine

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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Stx4-pmCitrine

Plasmid

#92422

Purpose

Expression of syntaxin-4 C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx4 (Stx4 Rat)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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Stx3-pmCitrine

Plasmid

#92421

Purpose

Expression of syntaxin-3 C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx3 (Stx3 Rat)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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Stx3-mCitrine-mCherry

Plasmid

#92426

Purpose

Expression of syntaxin-3 C-terminally conjugated to tandem of mCitrine and mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx3 (Stx3 Rat)

Use

Tags

mCherry and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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VAMP3-mCherry

Plasmid

#92423

Purpose

Expression of VAMP3 C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP3 (Vamp3 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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VAMP8-mCherry

Plasmid

#92424

Purpose

Expression of VAMP8 C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP8 (Vamp8 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

A36S (Please see Depositor Comments below)

Promoter

CMV

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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FKBP-Stx3-mCitrine

Plasmid

#92428

Purpose

Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx3 (Stx3 Rat)

Use

Tags

FKBP (FK506 binding protein 12); allows for heter…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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FKBP-Stx4-mCitrine

Plasmid

#92427

Purpose

Expression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx4 (Stx4 Rat)

Use

Tags

FKBP (FK506 binding protein 12); allows for heter…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO-sgRNA-puro

Plasmid

#104321

Purpose

3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

Depositor

Nathanael Gray

Article

Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1/TO

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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VAMP3(Δ71)-mCherry

Plasmid

#92425

Purpose

Expression of fusion incompetent VAMP3 (del71) C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP3 (Vamp3 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

deleted leucine 71

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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