We narrowed to 12,516 results for: NSI

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pcDNA3.1-VinculinI997A_TurboID_Venus

Plasmid

#200274

Purpose

Expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

VinculinI997A_TurboID_Venus

Expression

Mammalian

Mutation

change isoleucine to alanine at 997 of vinculin

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-BR1

Plasmid

#192816

Purpose

Expresses BR1 in bacteria

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

BR1 (BRI1 Mustard Weed)

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Silene_conica _GlnRS_MitoTP_pK7FWG2

Plasmid

#202654

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic GlnRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Silene_conica _LysRS_MitoTP_pK7FWG2

Plasmid

#202655

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic LysRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Agrostemma_githago _LysRS_MitoTP_pK7FWG2

Plasmid

#202656

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Agrostemma githago cytosolic LysRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Silene_conica _TyrRS_MitoTP_pK7FWG2

Plasmid

#202657

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic TyrRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Silene_vulgaris _TyrRS_MitoTP_pK7FWG2

Plasmid

#202658

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene vulgaris cytosolic TyrRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Silene_conica _PheRS_MitoTP_pK7FWG2

Plasmid

#202659

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to mito

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Silene_conica _PheRS_PlastidTP_pK7FWG2

Plasmid

#202660

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to plastid

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Arabidopsis_thaliana _PheRS_DualTP_pK7FWG2

Plasmid

#202661

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Arabidopsis thaliana PheRS known to be dual targeted to plastid and mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pT2h70CRET(#325)

Plasmid

#184060

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Hsp70-4

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-Ce25

Plasmid

#51296

Purpose

Expresses a plant arginyl-tRNA synthetase in E.coli

Depositor

Gabor Igloi

Article

Hogg et al Protein Expr Purif. 2008 Oct;61(2):163-7

Insert

arginyl-tRNA synthetase

Tags

His Tag and Thioredoxin

Expression

Bacterial

Mutation

GTT (Val) insertion after initiation codon to per…

Promoter

T7

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

Plasmid

#174890

Purpose

Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

U25C PylT

Promoter

EF1

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLNCX2 ER:ras

Plasmid

#67844

Purpose

4-hydroxytamoxifen inducible ER:ras fusion protein

Depositor

Masashi Narita

Article

Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

Insert

ER1a H-RasG12V fusion

Expression

Mammalian

Mutation

G525R renders the HBD largely insensitive to 17ß‐…

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-puro_FlnaABD-TS-16flag

Plasmid

#234868

Purpose

Expresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.

Depositor

Michael Murrell

Article

Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

Insert

FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16

Use

Lentiviral

Tags

GFP, RFP, FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-Neo-TRE-CMV-Cre-rtTA

Plasmid

#165457

Purpose

Tetracycline inducible CRE expression from AAVS1 locus

Depositor

Madeline Lancaster

Article

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

CRE

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pNvT-MHC::mCh

Plasmid

#67943

Purpose

Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI

Depositor

Ulrich Technau

Article

Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

mCherry

Use

Transgenic reporter in the sea anemone, nematoste…

Promoter

Myosin Heavy Chain1 (MyHC1)

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-MtAcKRS (human-opti)

Plasmid

#164195

Purpose

To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MtAcKRS-478(human-opti)

Use

Synthetic Biology

Expression

Mammalian

Mutation

D76G; L325M; L329I; Y330F; L333A; C372F

Promoter

CMV

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcdna DSG-2 Tailless

Plasmid

#89466

Purpose

DSG-2 tailless control

Depositor

Daniel Conway

Article

Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

Insert

desmoglein-2 (DSG2 Human)

Expression

Mammalian

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pOP/SSAT

Plasmid

#140291

Purpose

Expresses human SSAT in bacterial cells

Depositor

Saverio Bettuzzi, Gaetano Marverti

Article

Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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