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pDONR223_KRAS_p.G12A

Plasmid

#81673

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G12A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGV7 (CK2alpha' K69M, CK2beta)

Plasmid

#27095

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha' K69M (CSNK2A2 Human)

Use

Tetracycline-regulated expression

Tags

HA tag on CK2alpha' and Myc on CK2beta

Expression

Mammalian

Mutation

K69M on CK2alpha'

Promoter

bidirectional tet-responsive promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12F

Plasmid

#82807

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G12F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.D33E

Plasmid

#81659

Purpose

Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D33E

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-KRAS-A146V

Plasmid

#116422

Purpose

Lentiviral expression of KRAS A146V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

A146V

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAP3K6

Plasmid

#20519

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP3K6 (MAP3K6 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

Plasmid

#156416

Purpose

kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Use

Zebrafish transgenesis

Tags

mScarlet-I

Expression

Mutation

mutant KRAS4A-G12V

Promoter

kdrl

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP3K6

Plasmid

#23853

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K6 (MAP3K6 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_KRAS_p.G13V

Plasmid

#82800

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G13V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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KRAS-A146T

Plasmid

#154426

Purpose

A146T Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

Depositor

Kenneth Westover

Article

Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

Insert

KRAS (KRAS Human)

Use

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)

Plasmid

#180468

Purpose

kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

KRAS4A (KRAS Human)

Use

Zebrafish transgenesis

Tags

V5-mScarlet-I

Expression

Mutation

Promoter

kdrl

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)

Plasmid

#156406

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Use

Gateway cloning

Tags

mScarlet-I

Expression

Mutation

mutant KRAS4A-G12V

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

Plasmid

#156414

Purpose

kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4B-G12V (KRAS Human)

Use

Zebrafish transgenesis

Tags

EGFP

Expression

Mutation

mutant KRAS4B-G12V

Promoter

kdrl

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G13C

Plasmid

#82809

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G13C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.E62K

Plasmid

#81656

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E62K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12Y

Plasmid

#82799

Purpose

Gateway Donor vector containing KRAS , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G12Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)

Plasmid

#156413

Purpose

kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Zebrafish transgenesis

Tags

EYFP

Expression

Mutation

Promoter

kdrl

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

Plasmid

#156404

Purpose

Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12D (KRAS Human)

Use

Gateway cloning

Tags

mTagBFP2

Expression

Mutation

mutant KRAS4A-G12D

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

Plasmid

#159540

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12C

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X52-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169)

Plasmid

#159560

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) (KRAS Human)

Use

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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