Addgene

We narrowed to 586 results for: beta-actin

Showing: 341 - 360 of 586 results

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pB80-hKif5b(1-560)rigor-2xmNeongreen

Plasmid

#174649

Purpose

Live-cell marker for stable microtubules

Depositor

Lukas Kapitein

Article

Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

Insert

hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)

Use

Tags

mNeongreen-mNeongreen

Expression

Mammalian

Mutation

rigor mutation G234A

Promoter

Chicken beta-actin

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

Plasmid

#174645

Purpose

Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11

Use

Tags

SSPB(micro)-Venus and iLID-mCherry

Expression

Mammalian

Mutation

SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

Promoter

Chicken beta-actin

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)-L-GFP

Plasmid

#193716

Purpose

Expression of GFP tagged human kinesin-1 (KIF5B aa1-560)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)-L-GFP (KIF5B Human, Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-560);

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-383)-GFP-SSPB(milli)

Plasmid

#174626

Purpose

Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-383)-GFP-SSPB(milli) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(milli)

Expression

Mammalian

Mutation

mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-383)-GFP-SSPB(nano)

Plasmid

#174628

Purpose

Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(nano)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-383)-GFP-SSPB(nano) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(nano)

Expression

Mammalian

Mutation

mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del;

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-365)-GFP-SSPB(micro)

Plasmid

#174629

Purpose

Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF13A tail 361-1749

Plasmid

#162720

Purpose

Mammalian expression of WT mouse Kif13A with deletion of the 360 N-terminal residues

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Kif 13A (Kif13a Mouse)

Use

Tags

Expression

Mammalian

Mutation

WT with deletion of the 360 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBa.FRB-3myc-KIF13AWLDLE tail 361-1749

Plasmid

#224412

Purpose

Mammalian expression of KIF 13a WLDLE

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a WLDLE (Kif13a Mouse)

Use

Tags

FRB-3myc

Expression

Mammalian

Mutation

WLDLE

Promoter

Chicken Beta actin

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.FRB-3myc-KIF13AS1371A tail 361-1749

Plasmid

#224411

Purpose

Mammalian expression of KIF 13a S1371A

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371A (Kif13a Mouse)

Use

Tags

FRB-3myc

Expression

Mammalian

Mutation

S1371A

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-365)-mVenus-SSPB(micro)

Plasmid

#174635

Purpose

Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

mVenus-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-365)-GCN4-GFP-SSPB(micro)

Plasmid

#174632

Purpose

Highly processive KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-GCN4-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-KIF1A(1-383)-GFP-SSPB(micro)

Plasmid

#174627

Purpose

Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBT272_(pRosa26-GTET)

Plasmid

#36882

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

Plasmid

#36881

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-GluA1-scFV-Halo

Plasmid

#230065

Purpose

Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.

Depositor

Matthew Kennedy

Insert

Anti-GluA1 single chain variable fragment (scFv)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Chicken beta actin (CAG)

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pBa.NgCAM-mCherry

Plasmid

#180248

Purpose

Mammalian expression of NgCAM

Depositor

Marvin Bentley

Article

Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

Insert

NgCAM

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.NgCam.GFP

Plasmid

#45061

Depositor

Gary Banker, Marvin Bentley

Article

Burack et al Neuron. 2000 May;26(2):465-72.

Insert

NgCam (L1CAM Chicken)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Chicken Beta Actin

Available since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBLO44.5_PVYpro

Plasmid

#121634

Purpose

CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta actin promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO44.3_TuMVpro

Plasmid

#121632

Purpose

CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta actin promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBLO44.4_PPVpro

Plasmid

#121633

Purpose

CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta actin promoter

Available since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge