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pBTK501

Plasmid

#110602

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Available since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK519

Plasmid

#110603

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Available since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pGT-Ah7

Plasmid

#122574

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah10

Plasmid

#122577

Purpose

MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

tetQ/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK570

Plasmid

#110615

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pG1AK-mCherry

Plasmid

#71737

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Publication

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

mCherry

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Promoter

pRplsWT

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCM291rfp

Plasmid

#59775

Purpose

pCMrfp with TrfA mutation (291E to G)

Depositor

Nathan Hillson

Publication

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pDRAV-1

Plasmid

#26746

Depositor

Rolf Zeller

Publication

Osterwalder et al Nat Methods. 2010 Oct 17

Type

Empty backbone

Use

Cre/Lox; Flp/frt; phic31/attb-attp

Expression

Mammalian

Available since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pGT-Ah3

Plasmid

#122570

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRVdG-4BFP2

Plasmid

#52490

Purpose

Expresses mTagBFP2

Depositor

Ian Wickersham

Insert

mTagBFP2

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRVdG-4GCaMP6s

Plasmid

#52497

Purpose

Expresses GCaMP6s

Depositor

Ian Wickersham

Insert

GCaMP6s

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pBT316_pattB-pCA-GFP-pA

Plasmid

#52548

Purpose

for Phic31 integrase-mediated transgene integration

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pBLADE_ONLY_C

Plasmid

#168050

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

Depositor

Barbara Di Ventura

Publication

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Insert

Blue light-inducible AraC dimers in Escherichia coli

Expression

Bacterial

Promoter

J23101**

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTol2-Huc-Positron-ST

Plasmid

#129265

Purpose

pan neuronal expression of soma-localized Positron in zebrafish

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Zebrafish expression

Mutation

D92N, E199V

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pKD227

Plasmid

#90954

Purpose

Expresses S. baltica TtrS under the Ptac promoter

Depositor

Jeffrey Tabor

Publication

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

Tetrathionate sensor kinase

Expression

Bacterial

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR45Q

Plasmid

#92248

Purpose

HTT donor DNA with 45Q for homologous recombination-mediated gene modification

Depositor

Mahmoud Pouladi

Publication

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

HTT donor DNA (HTT Human)

Use

Precisionx hr targeting vector

Mutation

Barcode of wobble bases to prevent TALEN cleaving…

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mRuby3

Plasmid

#129257

Purpose

Express Ace2_D92N_E199V-mRuby3 in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mRuby3

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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U6GRNA

Plasmid

#68370

Purpose

gRNA scaffold with hU6 promoter

Depositor

Eric-Paul Bennett

Publication

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAC-EsaR-EsaI

Plasmid

#47660

Purpose

esaI gene downstream of esaR in pAC-EsaR (CmR)

Depositor

Cynthia Collins

Publication

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

esaI-term

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pCDFBB-eGFP

Plasmid

#32550

Depositor

Claudia Schmidt-dannert

Publication

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

egfp (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

Consitutive lac

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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