We narrowed to 9,752 results for: try

1. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

   Plasmid

   #127354

   Purpose

   gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, V5, and mVenus

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-GABARAPL1

   Plasmid

   #137759

   Purpose

   Expression of GABARAPL1

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   GABARAPL1 (GABARAPL1 Human)
   

   Tags

   HA and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. plentiCMV10x-sct-pp65-b2m-HLA-A0201

   Plasmid

   #196513

   Purpose

   Express single-chain pMHC with sequencing tag for 10x genomics

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   single chain of a pp65 peptide, b2m and HLA A0201
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Y84C, 10xgenomics compatible tag

   Promoter

   CMV

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. phage UbiC NLS HA stdMCP Halo

   Plasmid

   #104098

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   stdMCP-Halo
   

   Use

   Lentiviral

   Tags

   C-terminal Halo-tag, N-terminal NLS, and Two syno…

   Expression

   Mammalian

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST47-CYFIP1.GFP

   Plasmid

   #109139

   Purpose

   GFP-tagged CYFIP1 expression construct

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP1 (CYFIP1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDule-3-nitroTyrosine (5B)

   Plasmid

   #85498

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32H H70C D158S I159A L162R

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. VRK1_HUMAN_D0

   Plasmid

   #79684

   Purpose

   This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   VRK1 (VRK1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. HA-MAP1LC3A

   Plasmid

   #137756

   Purpose

   Expression of LC3A

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   MAP1LC3A (MAP1LC3A Human)
   

   Tags

   HA and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcBac1-Pyl-TAG

   Plasmid

   #228784

   Purpose

   Pyl aaRS/tRNA system for mammalian TAG suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   PylRS
   PytR
   

   Use

   Baculoviral

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA-mSin1(1.1)-HA

    Plasmid

    #73388

    Purpose

    Expresses mSin-HA

    Depositor

    Taekjip Ha, Jie Chen

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    mSin1 transcript variant protein isoform 1 (MAPKAP1 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

    Plasmid

    #127353

    Purpose

    gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and mVenus

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-betaPIXa

    Plasmid

    #15234

    Depositor

    Rick Horwitz

    Article

    Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

    Insert

    beta-PIXa (Arhgef7 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF160_VSV-G

    Plasmid

    #225963

    Purpose

    CMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-VSVG (env protein)
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-ECFP-betaPIXa

    Plasmid

    #15235

    Depositor

    Rick Horwitz

    Article

    Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

    Insert

    beta-PIXa (Arhgef7 Mouse)
    

    Tags

    ECFP and Flag

    Expression

    Mammalian

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pEGFPC1-ORAI1-EC-HA

    Plasmid

    #174335

    Purpose

    encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human Orai1 (ORAI1 Human)
    

    Tags

    GFP in the N-terminus and HA tag in the extracell…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSF-MjCouRS

    Plasmid

    #229066

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    Methanococcus jannaschii tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. p5E-dr-myl7-pro (JDW 1148)

    Plasmid

    #229836

    Purpose

    A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    cmlc2/myl7
    

    Use

    Gateway entry clone

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR221-TCF7

    Plasmid

    #79498

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    TCF7 (TCF7 Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid

    #127356

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

    Plasmid

    #127355

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and mVenus

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge