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Plasmid#70070PurposeGateway entry vector for human DND1 with stop codonDepositorInsertDnd1 (DND1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_GEC1
Plasmid#73945PurposeFor recombinant expression of human GEC1/GABARAPL1 in E. coliDepositorInsertGEC1 (GABARAPL1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S2-RAB_EKARev
Plasmid#160726PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.DepositorInsertS2-RAB_EKARev
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
VBS mutant of alpha catenin sensor
Plasmid#71710PurposeThis form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.DepositorInsertalpha-catenin ΔVBS FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 316-405 in α -catenin…PromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pR26-CMVconst-mBMP7
Plasmid#127375PurposeAllows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorInsertBMP7 (Bmp7 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMutationPromoterCMV enhancer/promoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDMX (1-120)
Plasmid#62065PurposeExpression of human MDM2 (1-120) in e. coliDepositorInsertMDM4 (MDM4 Human)
UseTagsGSTExpressionBacterialMutationcontains residues 1-120PromotertacAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S3-RAB_EKARev
Plasmid#160727PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.DepositorInsertS3-RAB_EKARev
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-FUSN-miRFP670-Cry2WT
Plasmid#122441PurposeExpresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertFUS (FUS Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationContains only amino acids 1-214PromoterSFFVAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-jRCaMP1a
Plasmid#160728PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-jRCaMP1a
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-Med25-VWA
Plasmid#64772PurposeMammalian expression of Flag-tagged Med25-VWADepositorInsertMed25-VWA (MED25 Human)
UseTagsFlagExpressionMammalianMutationC-terminal deletionPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF/S647A
Plasmid#74938PurposeMammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)DepositorInsertNSF (NSF Human)
UseTagsFLAGExpressionMammalianMutationS647A (mutation in the D2 domain of the protein)PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAED2
Plasmid#234599PurposeFluorescent reporter of the SOS responseDepositorInsertsmScarlet-I
gfp-mut2
UseReporterTagsExpressionBacterialMutationGFPmut2 was derived from avGFP with the following…PromoterPtet+dnaK P1 and cdaAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pMAL-MBP-FRB-KRas/N12
Plasmid#214302PurposeExpress split-KRas fragment fused with FRB and MBPDepositorInsertSplit-KRas fragment fused with FRB (KRAS Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-KRas/13C-FKBP-MBP
Plasmid#214303PurposeExpress split-KRas fragment fused with FKBP and MBPDepositorInsertSplit-KRas fragment fused with FKBP (KRAS Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-Q61LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR
Plasmid#207135PurposeDNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-inDepositorInsert5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 W391A pcDNA3
Plasmid#206085Purposeexpression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit bindingDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABD933
Plasmid#67917PurposeEntry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in plantaDepositorInsertCTG10
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF/T646A
Plasmid#74937PurposeMammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)DepositorInsertNSF (NSF Human)
UseTagsFLAGExpressionMammalianMutationT646A (mutation in the D2 domain of the protein)PromoterCMVAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF/T645A
Plasmid#74936PurposeMammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)DepositorInsertNSF (NSF Human)
UseTagsFLAGExpressionMammalianMutationT645A (mutation in the D2 domain of the protein)PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)
Plasmid#50914PurposeExpresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cellsDepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationP676C I730C N-term deletion of aa13-222 in hNKCC1…PromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V A735C (NT549)
Plasmid#50910PurposeExpresses human NKCC1 C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V A735C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847)
Plasmid#50865PurposeExpresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationA675C C723S C724V A735C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V V729C (NT543)
Plasmid#50907PurposeExpresses human NKCC1 C723S C724V V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V V729C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V W733C (NT547)
Plasmid#50909PurposeExpresses human NKCC1 C723S C724V W733C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V W733C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V F728C (NT542)
Plasmid#50906PurposeExpresses human NKCC1 C723S C724V F728C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V F728C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V M727C (NT540)
Plasmid#50905PurposeExpresses human NKCC1 C723S C724V M727C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V M727C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125/147L-siResist
Plasmid#49856PurposeEncodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationMethionine100, Methionine 125, and Methionine 147…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125L-siResist
Plasmid#49855PurposeEncodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationMethionine100 and Methionine 125 mutated to Leuci…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C820S (NT573)
Plasmid#49075PurposeExpresses human NKCC1 C820S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC820S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C1052S (NT674)
Plasmid#49076PurposeExpresses human NKCC1 C1052S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC1052S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C791S (NT572)
Plasmid#49074PurposeExpresses human NKCC1 C791S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC791S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C543M (NT360)
Plasmid#49068PurposeExpresses human NKCC1 C543M mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC543M in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S,C724V (NT501)
Plasmid#49073PurposeExpresses human NKCC1 C723S and C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC723S,C724V in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C630S (NT400)
Plasmid#49072PurposeExpresses human NKCC1 C630S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC630S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C630A (NT399)
Plasmid#49071PurposeExpresses human NKCC1 C630A mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC630A in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C295A (NT104)
Plasmid#49067PurposeExpresses human NKCC1 C295A mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC295A in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C295S (NT103)
Plasmid#49066PurposeExpresses human NKCC1 C295S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC295S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF2652
Plasmid#142969PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertARNTL2 (BMAL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB
Plasmid#122668PurposeC terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.DepositorInsertHNRNPA1 (HNRNPA1 Human)
UseLentiviralTagsmCherry-SspBExpressionMammalianMutationContains only amino acids 186-230PromoterSFFVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only