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pNHA8

Plasmid

#223650

Purpose

BccP protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BccP

Tags

MBP

Expression

Bacterial

Promoter

LacI

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA11

Plasmid

#223643

Purpose

Expression of ipgC-turboID fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

ipgC (ipgC )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA12

Plasmid

#223644

Purpose

Expression of turboID-ipgC fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

ipgC (ipgC )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA13

Plasmid

#223645

Purpose

Expression of mxiE-turboID fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

mxiE (mxiE )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA14

Plasmid

#223646

Purpose

Expression of turboID-mxiE fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

mxiE (mxiE )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNoT7

Plasmid

#218168

Purpose

pET31b plasmid with short insert lacking T7 and ribosome binding sites as control

Depositor

Emily Weinert

Article

Chauhan et al ACS Cent Sci. 2022 Nov 23;8(11):1518-152

Insert

Short sequence, mutated RBS and T7 sites

Expression

Bacterial

Promoter

NA - mutated T7

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM040_P8b_5'Ura4

Plasmid

#216461

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'ura4

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM036_P7_3"Ura4

Plasmid

#216465

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''ura4

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM024_P2_pMPA1

Plasmid

#216449

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter mpa1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM022_P2_pRPS1002

Plasmid

#216447

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rps1002

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM020_P2_pTRS1

Plasmid

#216445

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter trs1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM014_P2_pGPM1

Plasmid

#216439

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter gpm1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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Noodles Plasmid

Plasmid

#212613

Purpose

An all-in-one system for on-target efficiency analysis of CRISPR guide RNAs

Depositor

Ilya Vinnikov

Article

Lin et al MethodsX. 2023 Nov 15;12:102481. doi: 10

Type

Empty backbone

Use

CRISPR and Luciferase

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM042_P15678_MultiGeneBackbone

Plasmid

#216467

Purpose

Empty integration vector for genomic integration for multigene into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM041_P15678_SingleGeneBackbone

Plasmid

#216466

Purpose

Empty integration vector for genomic integration of 1 transcriptional unit into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM032_P4_tSynth30

Plasmid

#216457

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth30

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM031_P4_tSynth29

Plasmid

#216456

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth29

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM030_P4_tSynth27

Plasmid

#216455

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth27

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM029_P4_tSynth25

Plasmid

#216454

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth25

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM028_P4_tSynth3

Plasmid

#216453

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge