We narrowed to 4,489 results for: TIL;

1. pAAV-VTKS4-GCaMP6f

   Plasmid

   #178730

   Purpose

   AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   GCaMP6f
   

   Use

   AAV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKK-FLAG-TEV

   Plasmid

   #105768

   Purpose

   Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-VTKD6-dTom

   Plasmid

   #178717

   Purpose

   AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   dTom
   

   Use

   AAV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-VTKS4-dTom

   Plasmid

   #178718

   Purpose

   AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   dTom
   

   Use

   AAV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-VTKS5-dTom

   Plasmid

   #178719

   Purpose

   AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   dTom
   

   Use

   AAV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-VTKS6-dTom

   Plasmid

   #178720

   Purpose

   AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   dTom
   

   Use

   AAV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-VTKS4-GFP

   Plasmid

   #178712

   Purpose

   AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   GFP
   

   Use

   AAV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121816

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKK-TEV-mEGFP

   Plasmid

   #105794

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mEGFP

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1puro-shKIBRA-A

    Plasmid

    #40888

    Depositor

    Paul Reynolds

    Article

    Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

    Insert

    shKIBRA-A (WWC1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pLKO.1puro-shKIBRA-B

    Plasmid

    #40889

    Depositor

    Paul Reynolds

    Article

    Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

    Insert

    shKIBRA-B (WWC1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-CARMA2cl-FLAG

    Plasmid

    #97077

    Purpose

    Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 2 (CARD14 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL5.0 hCortKD-Flcortmut3YF EGFP

    Plasmid

    #187263

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Mutation

    3YF (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pE-cadherin-GFP-N-WASP deltaVCA

    Plasmid

    #101283

    Purpose

    Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

    Depositor

    Alpha Yap

    Article

    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

    Insert

    E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    delta VCA domain

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. ND4-FusXTBE-Left TALE

    Plasmid

    #196496

    Purpose

    Expresses left-side TALE-DddAtox Cterm half ND4-FusXTBE in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    COX8A MTS-ND4 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ND4-FusXTBE-Right TALE

    Plasmid

    #196497

    Purpose

    Expresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    COX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCVL.SSA TLR (CCR5 TALEN Sce Spacer)

    Plasmid

    #46941

    Purpose

    Codes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    5' iRFP arm
    eGFP with TALEN TS
    +3 mCherry
    3' iRFP arm
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    embedded TALEN TS from 163-218, truncated 25 amin…

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-3xNLS-OptoJNKi

    Plasmid

    #89740

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSS02:cyt-Peredox-mCherry

    Plasmid

    #161743

    Purpose

    cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

    Depositor

    Markus Schwarzländer

    Article

    Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

    Insert

    cyt-Peredox-mCherry
    

    Expression

    Plant

    Promoter

    Ubiquitin 10

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-B2UP49_GFP

    Plasmid

    #103071

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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