We narrowed to 13,644 results for: crispr cas9

1. pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188744

   Purpose

   Donor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188745

   Purpose

   Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   nonTATAchr21

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188746

   Purpose

   Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   nonTATAchrX

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19_CRISPR_DpmrA

   Plasmid

   #160903

   Purpose

   CRISPR_Cas9, lambda red recombineering

   Depositor

   Anne-Catrin Uhlemann

   Article

   McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

   Inserts

   Cas9
   Lambda Red Recombineering Genes
   Zeocin Resistance Cassette
   

   Promoter

   araBAD

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgCtr- LentiCRISPRv2

   Plasmid

   #107402

   Purpose

   Lentiviral expression of Cas9 and a control gRNA

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   control gRNA
   

   Use

   Lentiviral

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2-scr

   Plasmid

   #169795

   Purpose

   Expresses Cas9 and guide RNA with untarget sequence generated by scramble of the target sequence of LentiCRISPRv2-ACTB-C1..

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   Scrambled guide RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPRv2-ACTB-C1

   Plasmid

   #169796

   Purpose

   Expresses Cas9 and guide RNA for ACTB gene in human

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   guide RNA for ACTB gene
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR - CTRL sgRNA

   Plasmid

   #70662

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR - AAVS1 sgRNA

   Plasmid

   #70661

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against AAVS1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid

    #208647

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV and U6 in tandem

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid

    #208979

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV and U6 in tandem

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPRv2-Beclin1

    Plasmid

    #99574

    Purpose

    Expressed Cas9 with sgRNA targeting Beclin1

    Depositor

    Edward Campbell

    Article

    Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

    Insert

    gRNA targeting Beclin1 (BECN1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiCRISPRv2-KLF7-1

    Plasmid

    #169797

    Purpose

    Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for KLF7 neighbouring region
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiCRISPR-sgPREX1

    Plasmid

    #86127

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting PREX1

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    gRNA targeting PREX1 (PREX1 )
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiCRISPR-sgUFM1

    Plasmid

    #86133

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting UFM1

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA targeting UFM1 (UFM1 )
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPRv2-dADAM17 (#1)

    Plasmid

    #177259

    Purpose

    A knockout vector for the dog Adam17

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCRISPR-sgUFSP2

    Plasmid

    #86134

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting UFSP2

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA targeting UFSP2 (UFSP2 )
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPR-v1-sgMPC2_7

    Plasmid

    #163457

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 7 targeting GPT2 (MPC2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCRISPR-v1-sgMPC2_9

    Plasmid

    #163458

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 9 targeting GPT2 (MPC2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. TU#1806_CRISPR_unc-73_exon21

    Plasmid

    #82360

    Purpose

    to create unc-73E null allele

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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