We narrowed to 15,917 results for: genome
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Plasmid#122507Purposeexpresses WT spCas9 and a chimeric gRNA targeting human LMNADepositorInsertLMNA-gRNA1
UseCRISPRExpressionMammalianPromoterU6Available SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9-T2A-GFP
Plasmid#53191PurposeCo-expresses human optimized S. pyogenes dCas9 and GFPDepositorInserthumanized dead Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailable SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
4xNLS-pMJ915v2
Plasmid#88917PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRExpressionBacterialAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131A
Plasmid#69273PurposeGolden Gate entry vector; 1st gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS2
Plasmid#196978PurposeExpresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS2 tag
ExpressionPlantPromoterpG10-90Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEff
Plasmid#97423PurposeTransient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFD1-dU6:1gRNA
Plasmid#49408Purposeexpression of gRNA under control of the Drosophila U6:1 promoterDepositorInsertdU6-1:gRNA
UseCRISPRExpressionInsectPromoterdU6-1Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ150
Plasmid#69301PurposeGateway entry vector with pcoCas9 (Plant codon optimized)DepositorInsertpcoCas9 (plant codon-optimized)
UseCRISPRTags3XFLAGMutationNLS fusionAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
PABE-7
Plasmid#115628PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-nCas9-3*NLS
UseCRISPRExpressionPlantAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pADH100
Plasmid#90980PurposeNAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-BE1
Plasmid#73018PurposeExpresses BE1 with N-terminal His6 tag in E. ColiDepositorInsertBE1
TagsHis6 tagExpressionBacterialPromoterT7Available SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W
Plasmid#112927PurposeCRISPR-a reporter assay lentiviral vector (gTetO)DepositorInserthU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p415-GalL-Cas9-CYC1t
Plasmid#43804PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRExpressionYeastPromoterGalLAvailable SinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
phL1A-pcDNA3
Plasmid#12307DepositorInsertL1CAM (L1CAM Human)
ExpressionMammalianAvailable SinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W
Plasmid#112926PurposeCRISPR-a reporter assay lentiviral vector (empty gRNA control)DepositorInserthU6-gRNASAM(empty), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_Kdm5b_MUT
Plasmid#119767Purposein vitro transcription for mRNADepositorInsertKdm5b MUT
Available SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK148-Pnmt41-GBP-mCherry(C)-leu1+
Plasmid#89069Purposegenomic GBP-mCherry epitope C-terminal tagging at leu1+ locus in S. pombeDepositorTypeEmpty backboneUseGenomic integration in s. pombeTagsGBP-mCherryExpressionYeastAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
p201N Cas9
Plasmid#59175PurposeCas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
nptII
UseCRISPRPromoter2x35S and StUbi-3PAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS1
Plasmid#196977PurposeExpresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS1 tag
ExpressionPlantPromoterpG10-90Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only