We narrowed to 3,867 results for: mak

1. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

   Plasmid

   #195741

   Purpose

   Base editing for making a pre-stop codon in mmoX using phenol inducible system

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Po (phoenol-inducible promoter)

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-HA-Akt1-K179M

   Plasmid

   #73409

   Purpose

   Expresses kinase-inactive mutant of Akt with HA tag

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   changed K179M to make Kinase inactive mutant

   Promoter

   CMV

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIVT/CamKII gamma CA

   Plasmid

   #122142

   Purpose

   For making constitutively active CamKII gamma RNA

   Depositor

   Carmen Williams

   Article

   Miao et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   constitutively active CamKII gamma (Camk2g Mouse)
   

   Use

   For in vitro transcription

   Mutation

   First 291 amino acid residues of CaMKII gamma

   Promoter

   T7

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4/TO/GFP-ZNF598

   Plasmid

   #141191

   Purpose

   Expresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

   Insert

   ZNF598 (ZNF598 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-mCardinal-P2A-B19L Flp-in vector

   Plasmid

   #178519

   Purpose

   For making TRE-CB mouse line in Tigre locus

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   mCardinal-P2A-B19L
   

   Expression

   Mammalian

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

   Plasmid

   #90015

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Ago2
   NBirA*-GFP
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer

   Plasmid

   #90013

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Ago2
   NBirA*-Dicer
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CaMKIV-dCT

   Plasmid

   #126422

   Purpose

   Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

   Depositor

   Douglas Black

   Article

   Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

   Insert

   Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

   Plasmid

   #178518

   Purpose

   For making Rabies virus SAD B19 strain L cell line with hyPBase

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   B19L-IRES-mCherry
   

   Expression

   Mammalian

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

    Plasmid

    #90010

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C WT
    NBirA*-14-3-3e
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJC5-4-insulator-BREreporter-insulator

    Plasmid

    #171499

    Purpose

    injections to make Bmp reporter mouse transgenic line

    Depositor

    James Briscoe

    Article

    Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

    Insert

    Insulator BRE-MLP:H2B-Turqoise
    

    Expression

    Mammalian

    Promoter

    BRE-MLP

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

12. pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)

    Plasmid

    #129667

    Purpose

    A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBF_HsPepT1

    Plasmid

    #73943

    Purpose

    Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag

    Depositor

    Simon Newstead

    Article

    Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

    Insert

    PepT1 (SLC15A1 Human)
    

    Use

    Make rna for xenopus oocyte injection

    Tags

    Flag

    Promoter

    beta globin

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pBF_HsPepT2

    Plasmid

    #73944

    Purpose

    Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag

    Depositor

    Simon Newstead

    Article

    Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

    Insert

    PepT2 (SLC15A2 Human)
    

    Use

    Make rna for xenopus oocyte injection

    Tags

    Flag

    Promoter

    beta globin

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pICE-HA-CtIP-siR-WT

    Plasmid

    #82030

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mutations making the cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-Blast PhLP1-myc-6xHis

    Plasmid

    #218178

    Purpose

    Used to make a lenti virus for stable expression of phosducin-like protein 1 in mammalian cells

    Depositor

    Barry Willardson

    Article

    Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

    Insert

    Phosducin like protein 1 (PDCL Human)
    

    Use

    Lentiviral

    Tags

    6x His and Myc

    Expression

    Mammalian

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMHE-NLS-Beta-catenin-S33Y

    Plasmid

    #138537

    Purpose

    For making Beta-catenin-S33Y RNA

    Depositor

    Carmen Williams

    Article

    Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

    Insert

    Beta-catenin
    

    Use

    In vitro transcription

    Tags

    2XFLAG and 2xNLS

    Mutation

    changed serine 33 to tyrosine

    Promoter

    T7

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

    Plasmid

    #90014

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-TNRC6C
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYL236

    Plasmid

    #173184

    Purpose

    CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 (EZH2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP

    Plasmid

    #90012

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C
    NBirA*-GFP
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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