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Showing: 3621 - 3640 of 8782 results

  1. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

    Plasmid
    #189636
    Purpose
    Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries
    Depositor
    Manuel Kaulich
    Article
    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.
    Insert
    hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    189636-plasmid-map-sequence-id-414907

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  7. ATP1A1_G6_T804N_Std_Dual_epegRNA_tevopreQ1

    Plasmid
    #187457
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187457-plasmid-map-sequence-id-367465

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  12. pINSPECT_SigP:NLuc

    Plasmid
    #197263
    Purpose
    Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197263-plasmid-map-sequence-id-389724

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  17. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  18. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

    Plasmid
    #140020
    Purpose
    Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…
    Promoter
    EF1
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140020-plasmid-map-sequence-id-297657

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  19. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available Since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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  20. AICSDP-34: TNNI1-mEGFP

    Plasmid
    #114411
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114411-plasmid-map-sequence-id-220877

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Showing: 3621 - 3640 of 8782 results