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Nrp2.5-AP-His

Plasmid

#71976

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.5 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.4-AP-His

Plasmid

#71975

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.4 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Ntng1.j-AP-His

Plasmid

#71993

Purpose

Expresses the extracellular region of the Netrin G1, isoform j protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntng1.j (Ntng1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARF1-no stop

Plasmid

#67307

Purpose

hArf1 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pGL2-HKII-B

Plasmid

#64514

Purpose

AS-30D hepatoma hexokinase II 3.0 kbp promoter-luciferase reporter vector

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

Insert

Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)

Use

Luciferase

Tags

firefly luciferase

Mutation

Isolated from AS-30D rat hepatoma

Promoter

Hexokinase II

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pGL2-HKII-F

Plasmid

#64518

Purpose

AS-30D hepatoma hexokinase II 0.075 kbp exon I-luciferase reporter vector

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

Insert

Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)

Use

Luciferase

Tags

firefly luciferase

Mutation

Isolated from AS-30D rat hepatoma

Promoter

Hexokinase II promoter deleted

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
pScalps_puro_mIL-2Rα ST

Plasmid

#59919

Purpose

Expresses mouse interleukin-2 receptor alpha chain mutated

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Mutation

changed sequence of the intracellular (C-terminal…

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
psicheck-2-Hsdl2-mod

Plasmid

#48180

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of HSDL2 (Hsdl2 Mouse)

Use

Luciferase

Mutation

A/T MUTATION IN ATG OF UORF

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Hsdl2

Plasmid

#48179

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of HSDL2 (Hsdl2 Mouse)

Use

Luciferase

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
pmyc-GFP-TNRC6A-del.GW-II+III-NES-mut

Plasmid

#42043

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

deleted aa716-772 and aa804-924, F951A, F955A, I9…

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-del.GW-I+III-NES-mut

Plasmid

#42042

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

deleted aa457-532 and aa804-924, F951A, F955A, I9…

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-del.GW-I+II-NES-mut

Plasmid

#42039

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

deleted aa457-532 and aa716-772, F951A, F955A, I9…

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-del.GW-III-NES-mut

Plasmid

#42041

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

deleted aa804-924, F951A, F955A, I958A, F960A

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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Abr GAP domain R683A N795A/pGEX 3X

Plasmid

#36408

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Abr GAP domain with R683A N795A (ABR Human)

Tags

GST

Expression

Bacterial

Mutation

R683A and N795A

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1Xpress-AbrR683AN795A-HisC

Plasmid

#32510

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

ABR (ABR )

Tags

HISC

Expression

Mammalian

Mutation

R 683 to A and N 795 to A

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
integrin beta6 777T pBS

Plasmid

#13581

Depositor

Dean Sheppard

Article

Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

Insert

integrin beta 6 777T (ITGB6 Human)

Expression

Bacterial

Mutation

777T. Lacks last 11 amino acids of cytoplasmic do…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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tet-pLKO-sgRNA-puro

Plasmid

#104321

Purpose

3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

Depositor

Nathanael Gray

Article

Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1/TO

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Rtn4a-GFP

Plasmid

#61807

Purpose

Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Rtn4a (RTN4 Human)

Tags

AcGFP

Expression

Mammalian

Mutation

Rtn4a Stop Codon removed

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLV_3XHA-ATP6V1B2-mNeonGreen

Plasmid

#228865

Purpose

Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V1B2 (ATP6V1B2 Human)

Use

Lentiviral

Tags

3xHA and mNeonGreen

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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NC15 pGLUE CUL3

Plasmid

#36970

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

CUL3 (CUL3 Human)

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only