We narrowed to 11,503 results for: NSI

1. pET3a-AqAdk_Y52C

   Plasmid

   #18090

   Depositor

   Dorothee Kern

   Article

   Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

   Insert

   adenylate kinase (kad A. aeolicus)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Tyr 52 changed to Cys

   Promoter

   Available since

   May 29, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pDONR_P2R-P3_3'arm_apobb.1

   Plasmid

   #128681

   Purpose

   apobb.1 homology arm 3' gateway clone

   Depositor

   Steven Farber

   Article

   Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

   Insert

   apobb.1 (apobb.1 Zebrafish)
   

   Use

   Multi-step gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJUMP47ts-2A(sfGFP)

   Plasmid

   #126992

   Purpose

   Level 2 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   RepA A56V thermosensitive mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRL1058

   Plasmid

   #70686

   Purpose

   Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.

   Depositor

   Peter Wolk

   Article

   Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

   Insert

   Transposon Tn5-1058
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. YCe1901 HC_Kan_PGKpA_p22

   Plasmid

   #100683

   Purpose

   part designed to occupy position 22 of EMMA. Functional category: polyadenylation signal sequence

   Depositor

   Yizhi Cai

   Article

   Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

   Insert

   PGKpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET26-CNTN149S/F366A

   Plasmid

   #100165

   Purpose

   recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

   Depositor

   Seok-Yong Lee

   Article

   Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

   Insert

   Concentrative nucleoside transporter
   

   Use

   Tags

   MBP-His

   Expression

   Bacterial

   Mutation

   N149S, F366A

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Flag-YAP-P85A

   Plasmid

   #33062

   Depositor

   Kunliang Guan

   Article

   Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   P85A

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pBT255_(pCA-GFP4m)

   Plasmid

   #36877

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F64L; S65T; V163A; S175G

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS314-PRP16

   Plasmid

   #89971

   Purpose

   Prp16 in a trp backbone complements prp16 genomic deletion

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP16 (PRP16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   none

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pL-rpb1-C67Y

    Plasmid

    #10982

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-C67Y (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 200 G to A in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pL-rpb1-C70Y

    Plasmid

    #10983

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-C70Y (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 209 G to A in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pL-rpb1-H80Y

    Plasmid

    #10984

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-H80Y (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 238 C to T in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

13. LexAop2->Bxb1.STOP>myr::4xSNAPf

    Plasmid

    #87640

    Purpose

    For creating drosophila transgenics expressing LexAop2->Bxb1.STOP>myr::4xSNAPf

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    myr::4xSNAPf
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDESTFLAGHApuro3-Ago Chi-a

    Plasmid

    #14075

    Depositor

    Thomas Tuschl

    Article

    Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

    Insert

    Ago Chi-a
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Ago Chimera-a

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pDZ529 pTRP MET25p PP7-PS-2x-yeGFP

    Plasmid

    #72233

    Purpose

    Tryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    PP7-PS-2x-yeGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDESTFLAGHApuro3-Ago Chi-b

    Plasmid

    #14085

    Depositor

    Thomas Tuschl

    Article

    Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

    Insert

    Ago Chi-b
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Ago Chimera-b

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pDESTFLAGHApuro3-Ago Chi-c

    Plasmid

    #14086

    Depositor

    Thomas Tuschl

    Article

    Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

    Insert

    Ago Chi-c
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Ago Chimera-c

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

18. p6671 MSCV-IP N-HAonly 38E6

    Plasmid

    #44144

    Depositor

    Peter Howley

    Article

    White et al J Virol. 2012 Dec;86(24):13174-86. doi:

    Insert

    HPV38E6
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pLL3.7 Alk.1 shRNA

    Plasmid

    #59296

    Purpose

    shRNA to mouse Alk

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

    Insert

    Alk.1 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCSP1-HA-xEcto

    Plasmid

    #20900

    Depositor

    Stefano Piccolo

    Article

    Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

    Insert

    Ectodermin (trim33 Frog)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    contains full unsequenced 3' UTR

    Promoter

    Available since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only