We narrowed to 63,044 results for: cloning

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pLentiPGK Hygro DEST ERKKTRmCerulean3

Plasmid

#90229

Purpose

Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Publication

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-BE-zCas9

Plasmid

#101739

Purpose

expression of an optimized Cas9 for base-editing in zebrafish

Depositor

Shuo Lin

Publication

Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

Insert

rAPOBEC1-XTEN-zCas9-UGI-NLS

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

CMV

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGPTVII-Bar-U-MatryoshCaMP6s

Plasmid

#100024

Purpose

Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Publication

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Expression

Plant

Promoter

AtUBQ10

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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MED12_pLX307

Plasmid

#98350

Purpose

Lentiviral expression of MED12

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MED12 (MED12 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R1392Q

Promoter

E1Fa

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223_VHL_WT

Plasmid

#81874

Purpose

Gateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223_HIF1A_WT

Plasmid

#82129

Purpose

Gateway Donor vector containing HIF1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIF1A (HIF1A Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pet28a-His6-Halo-TEV-Nrf2

Plasmid

#62455

Purpose

Halo-His6 tagged Nrf2 for E.coli expression

Depositor

Yimon Aye

Publication

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Nrf2 (NFE2L2 Human)

Tags

His6-Halo-TEV

Expression

Bacterial

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-PR50

Plasmid

#84905

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Publication

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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piRFP-N3-Tyrosinase

Plasmid

#80152

Purpose

Melanosome marker

Depositor

Santiago Di Pietro

Publication

Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

Insert

Tyrosinase (TYR Human)

Tags

iRFP682

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pRS315e_pGal-nCas9(D10A)-PmCDA1

Plasmid

#79617

Purpose

Expresses nCas9(D10A)-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Publication

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pclbw-opa1(isoform 1)-myc

Plasmid

#62845

Purpose

Mammalian Expression of myc-tagged Opa1 isoform1

Depositor

David Chan

Publication

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mmOPA1isoform1-myc (Opa1 Mouse)

Use

Retroviral

Tags

9xMyc

Expression

Mammalian

Promoter

cmv-bactin

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pT2SC

Plasmid

#59382

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

Depositor

Shelley Copley

Publication

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-cat

Use

Template

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCFJ910

Plasmid

#44481

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, MCS

Use

Multiple cloning site vector

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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tetO-Myocd

Plasmid

#46033

Depositor

John Gearhart

Publication

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

Myocd (Myocd Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-C 18E7

Plasmid

#37886

Depositor

Karl Munger

Publication

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 18 E7 (E7 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pLemiR-NS

Plasmid

#32809

Depositor

Jerry Crabtree

Publication

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

non-specific hairpin

Use

Lentiviral

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223-DDR2

Plasmid

#23897

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DDR2 (DDR2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pLenti X2 Blast DEST (w46-2)

Plasmid

#17391

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Blast

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning, Lentiviral, and RNAi

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pgLAP2

Plasmid

#19703

Depositor

Peter Jackson

Type

Empty backbone

Tags

Flag, S peptide, and TEV

Expression

Mammalian

Available since

Feb. 19, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFRT/FLAG/HA-DEST EIF2C1

Plasmid

#19887

Depositor

Thomas Tuschl

Publication

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Eukaryotic translation initiation factor 2C, 1 (AGO1 Human)

Tags

FLAG/HA

Expression

Mammalian

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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