We narrowed to 59,428 results for: cloning
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Plasmid#100709PurposeB52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorInsertsgSTOP targeting SPRTN (cloned using BbsI)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA
Plasmid#121750PurposepMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsert3x HA epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-nsp1-c3xFLAG
Plasmid#215694PurposeProduces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp1
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNAintron-CHIKV-nsp2-c3xFLAG
Plasmid#215695PurposeProduces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp2
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNAintron-CHIKV-nsp4-c3xFLAG
Plasmid#215697PurposeProduces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp4
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
IG510: pMVP (R4-R3) human PDX1
Plasmid#121744PurposepMVP R4-R3 entry plasmid, contains human PDX1 for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertHuman PDX1 (PDX1 Human)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV L1-R5
Plasmid#62090PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorExpressionMammalianAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 L5-L2
Plasmid#62095PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and multiple cloning site module. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorExpressionMammalianAvailable SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
KK501: pMVP (L3-L2) FKBP DD + polyA
Plasmid#121789PurposepMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.DepositorInsertFKBP Degron Domain + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CCL5.Pro
Plasmid#79492PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoterDepositorInsertCCL5 Promoter (CCL5 Human, Synthetic)
UseMultisite gateway entry clonePromoterCCL5 PromoterAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS
Plasmid#121835PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEXNKI-GST-3C-LIC
Plasmid#108710PurposeExpression of a GST-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsGST-3C protease cleavage siteExpressionBacterialPromotertacAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1
Plasmid#121824PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9/LSD1 (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IX301: pMVP (L3-L2) myc epitope tag + polyA
Plasmid#121753PurposepMVP L3-L2 entry plasmid, contains myc epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsertmyc epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-TAZ CA
Plasmid#79504PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDSDepositorInsertWWTR1 (WWTR1 Human)
UseMultisite gateway entry cloneTags3xFLAGMutationS89APromoterno PromoterAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)
Plasmid#154838PurposeInsertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selectionDepositorInsertsynthetic guide::∆HYGR::unc-54 3' UTR
UseCRISPR and Cre/LoxExpressionWormMutation∆HYGR is promoterless and encodes aa 60-341Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
IX601: pMVP (L3-L2) FLAG epitope tag + polyA
Plasmid#121751PurposepMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsertFLAG epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ401: pMVP (L3-L2) P2A-Neo + polyA
Plasmid#121781PurposepMVP L3-L2 entry plasmid, contains Neo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to gene of interest.DepositorInsertP2A-Neo + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ601: pMVP (L3-L2) P2A-Zeo + polyA
Plasmid#121783PurposepMVP L3-L2 entry plasmid, contains Zeo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to gene of interest.DepositorInsertP2A-Zeo + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ901: pMVP (L3-L2) P2A-eGFP + WPRE
Plasmid#121772PurposepMVP L3-L2 entry plasmid, contains eGFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest in lentivirus vectors.DepositorInsertP2A-eGFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only