We narrowed to 14,764 results for: nts

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miniTn7(Gm)PA1/04/03-ecfp-a

Plasmid

#111619

Purpose

eCFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-ecfp

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLO99

Plasmid

#90219

Purpose

Complements mutations in Aspergillus nidulans gene lysB (AN5206) (mutants require lysine)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_03691

Use

Aspergillus

Promoter

pLac

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMC-J6-dCas9-10XGP41-J9

Plasmid

#202005

Purpose

MoClo level 0 dCas9-10XGP41 coding sequence module

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Insert

dCas9-10XGP41

Use

Synthetic Biology; Moclo cloning vector

Tags

GS linkers

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG

Plasmid

#158127

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET16_Caspase-4_p10

Plasmid

#214308

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_p20_C258A

Plasmid

#214307

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

C258A

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 in pENTR

Plasmid

#216585

Purpose

gateway cloning donor vector containing EEF1A2

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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DDX3X in pENTR

Plasmid

#216572

Purpose

gateway cloning donor vector containing DDX3X

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway entry plasmid

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL701

Plasmid

#140371

Purpose

Expresses TetR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-TetR-T

Tags

6XHis

Expression

Bacterial

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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YeNL_pRSETb

Plasmid

#89520

Purpose

Yellow color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPBpuro-4493NES(Booster-PKA)

Plasmid

#184709

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSBLego

Plasmid

#219967

Purpose

The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.

Depositor

Markus Seeger

Article

Ackle et al Sci Rep. 2025 Mar 19;15(1):9460. doi: 10

Type

Empty backbone

Tags

SLEHHHHHH and pelB signal sequence

Expression

Bacterial

Promoter

pBAD

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTRANS_212

Plasmid

#91111

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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GeNL(Ca2+)_520/pcDNA3

Plasmid

#85204

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_520

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A3001

Plasmid

#91042

Purpose

Module A, Promoter: 35S, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

35S

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTW170b2

Plasmid

#136929

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gVI

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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Skylan-S-(GGGGS)x3-β-Actin

Plasmid

#128938

Purpose

fluorescnece reporter of β-Actin with Skylan-S. Express in mammalian cells

Depositor

Shimon Weiss

Article

Yi et al Biomed Opt Express. 2019 Apr 16;10(5):24

Insert

Skylan-S

Tags

β-actin

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PEP109E

Plasmid

#97395

Purpose

binary construct of cytosolic mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

cytosol

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_K356D

Plasmid

#214312

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

K356D

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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R4227X-F

Plasmid

#21371

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG (following D4226)

Expression

Mammalian

Mutation

Changed R4227 to stop codon

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only