We narrowed to 5,482 results for: PID;

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pAAV-EF1a-DIO-JEDI-1P-WPRE

Plasmid

#202611

Purpose

Double floxed genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-AgDD

Plasmid

#80625

Purpose

create nuclear protein aggregates in mammalian cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NLS-NES-L106P-sfGFP (FKBP1A Human)

Tags

MPPKKKRKVLALKLAGLDI

Expression

Mammalian

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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CD40L-MIEG-hCD4 (hCD4-CD40L)

Plasmid

#40355

Depositor

Alexander Dent

Article

Kusam et al Int J Cancer. 2009 Aug 15;125(4):977-81.

Insert

CD40L (Cd40lg Mouse)

Use

Retroviral

Tags

IRES hCD4

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pCSIIbsr-Eevee-ROCK-NES

Plasmid

#209918

Purpose

A FRET biosensor for ROCK activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

Eevee-ROCK-NES

Use

Lentiviral

Tags

ECFP and YPet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Tom20-CR

Plasmid

#171461

Purpose

Expresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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Opto-casp9_AtCRY2-PHR-casp9

Plasmid

#131221

Purpose

Expresses Opto-casp9_AtCRY2-PHR-casp9 (light-activated caspase9)

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

Opto-casp9 (AtCRY2-PHR-casp9)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFP

Plasmid

#199366

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOEu-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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AA184

Plasmid

#216047

Purpose

Fragmid fragment: (C' terminus) for prime editing; using PEmax

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

SV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA058

Plasmid

#216040

Purpose

Fragmid fragment: (C' terminus) for prime editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

MMLV-RT_v1.1; SV40NLS_v1.3

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-PLA2G15-6xHis S198A

Plasmid

#213605

Purpose

Expresses PLA2G15-6xHis S198A in mammalian cells

Depositor

Monther Abu-Remaileh

Article

Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

Insert

Phospholipase A2 Group XV (PLA2G15 Human)

Tags

6xHis

Expression

Mammalian

Mutation

Changed serine 198 to alanine

Promoter

CMV

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV Renilla Luciferase L5-L2

Plasmid

#62186

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Renilla luciferase

Use

Luciferase; Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-DDX4LCD

Plasmid

#204408

Purpose

E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

DDX4(1-236) (DDX4 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMuLE Lenti Dest Neo

Plasmid

#62178

Purpose

MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Lentiviral; Mule gateway destination vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCambia2300 - ProRUBY:RUBY-Citrine

Plasmid

#128796

Purpose

Fluorescent reporter for subcellular localisation and expression analysis of RUBY

Depositor

George Haughn

Article

Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

Insert

RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)

Tags

Citrine

Expression

Plant

Promoter

ProRUBY

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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OPEN-A*24:02

Plasmid

#235133

Purpose

E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*24:02 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*01:01

Plasmid

#235134

Purpose

E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*01:01 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P.1

Plasmid

#177959

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )

Expression

Mammalian

Mutation

P80R,R203K,G204R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-53BP1LCD

Plasmid

#204407

Purpose

E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

53BP1(1208-1977) (TP53BP1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP.N1-HDAC6.delta Buz

Plasmid

#36190

Depositor

Tso-Pang Yao

Article

Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

Insert

HDAC6..delta Buz (HDAC6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the C-terminal ub binding domain (BUZ…

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pMK284 (Stag-3FLAG-Hygro)

Plasmid

#72800

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Hygro

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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