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pAPcrRNA_Lmo

Plasmid

#126501

Purpose

L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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eNme2-C TadCBEa

Plasmid

#193840

Purpose

Express TadCBEa (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

eNme2-C TadCBEa

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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EK0423 SFFV-mTagBFP2-SGc(s70587)cSG-mCherry (FLP-IN)

Plasmid

#191162

Purpose

Expression of a sensor for synthetic sequence 1 (EK0208) in mammalian cells; mTagBFP2 marker, mCherry output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mTagBFP:s70587:mCherry

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p6898 PHAGE-P CMVT N-HA-hE6AP I C820A

Plasmid

#37602

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP I C820A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C820A

Promoter

CMV tet regulatable

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
3540 pMIG Bid p15

Plasmid

#8789

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bid p15 (Bid Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
P55.SRE.mCMV.flag.mSrtA-3M.wpre

Plasmid

#226774

Purpose

secretory SortaseA in p53 (SRE backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

sortase A (srtA )

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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EK0325 SFFV-mCherry-SG(EGFP.s2)SG-Cre (FLP-IN)

Plasmid

#191160

Purpose

Expression of a sensor for EGFP CDS in mammalian cells; mCherry marker, Cre output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:EGFP.s2:Cre

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLmoCascade-IL1RNcrRNA

Plasmid

#126487

Purpose

Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Lmo IL1RN cr03

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSicoR-TMF1i2

Plasmid

#59625

Purpose

Expression of shRNA against human TMF1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

TMF1 (TMF1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-TMF1i1

Plasmid

#59624

Purpose

Expression of shRNA against human TMF1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

TMF1 (TMF1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
3538 pMIG Bad

Plasmid

#8787

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bad (Bad Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 19, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA-h-mmPtch1-FL-TagBFP

Plasmid

#120921

Purpose

Expresses BFP tagged Ptch1

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSI-535

Plasmid

#131130

Purpose

gRNA expression vector for C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

C-to-T reporter activation gRNA

Use

CRISPR

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX2T merlin 1-332

Plasmid

#11631

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino-terminal half of merlin.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
ch-Tog

Plasmid

#28290

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

ch-TOG (CKAP5 Human)

Expression

Bacterial and Mammalian

Mutation

K19R, E118G

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only

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pKmyc-Borg3

Plasmid

#141074

Purpose

Expresses Borg3 (Cdc42ep5) in mammalian cells

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol. 2001 Oct;3(10):861-6. doi

Insert

Borg3 (Cdc42ep5 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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TDP43 NOTAG3

Plasmid

#28208

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Expression

Mammalian

Mutation

aa170-414 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM29i1

Plasmid

#59606

Purpose

Expression of shRNA against human ADAM29

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM29 (ADAM29 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pTXB3 AurA

Plasmid

#68270

Purpose

bacterial expression of human AurA

Depositor

Stefano Ferrari

Article

Krystyniak et al Oncogene. 2006 Jan 19;25(3):338-48.

Insert

AurA (AURKA Human)

Tags

Mxe intein/chitin binding domain

Expression

Bacterial

Mutation

See depositor comment below

Promoter

T7

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pQZ083

Plasmid

#106417

Purpose

Express Complexin-1 fragment (1-83) in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Insert

complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)

Tags

GST tag

Expression

Bacterial

Mutation

Fragment 1-83, Codon optimization

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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