We narrowed to 21,062 results for: CRISPR-Cas9

1. pRRL-gRNA(anti-cGAS)-Cas9-Puro

   Plasmid

   #130908

   Purpose

   gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

   Plasmid

   #192131

   Purpose

   Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-Hsp2Cas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

   Plasmid

   #169596

   Purpose

   Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-SpCas9 hygro

   Plasmid

   #104995

   Purpose

   This lentiviral construct delivers hSpCas9 and hygromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-SpCas9 neo

   Plasmid

   #104996

   Purpose

   This lentiviral construct delivers hSpCas9 and G418 resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-SpCas9 puro

   Plasmid

   #104994

   Purpose

   This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-SpCas9 blast

   Plasmid

   #104997

   Purpose

   This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

   Plasmid

   #185913

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

   Plasmid

   #188703

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

    Plasmid

    #101104

    Purpose

    Encodes dCas9(N) fused to VEGFR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

    Plasmid

    #185918

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101114

    Purpose

    Encodes dCas9(N) fused to CXCR4

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    CXCR4-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE

    Plasmid

    #101106

    Purpose

    Encodes FKBP-dCas9(N) fused to VEGFR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR2-FKBP-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

    Plasmid

    #227773

    Purpose

    Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    Cas9-t2A-GFP
    4xU6:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and tp1

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

    Plasmid

    #227774

    Purpose

    Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Insert

    loxP-dsR-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid

    #221503

    Purpose

    CRISPRi machinery fish codon optimized expressing plasmid

    Depositor

    Alessandro Ori

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    D10A and H839A point mutations

    Promoter

    Zebrafish Ubiquitin promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pnos-Fok1:dCas9-nos

    Plasmid

    #62210

    Purpose

    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Insert

    Fok1-dCas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    nanos

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAS9PBKS

    Plasmid

    #68371

    Purpose

    CbAkt promoter driven Cas9-2A-GFP (from px458)

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Insert

    Cas9
    

    Use

    Tags

    2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CbAkt

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. NmeCas9_3XNLS_pMCSG7

    Plasmid

    #120078

    Purpose

    Bacterial expression of wtNmeCas9 with 3X NLS

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    NmeCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti_dNmeCas9

    Plasmid

    #120077

    Purpose

    dNmeCas9 with no sgRNA cassette lenti construct

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    dNmeCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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