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pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183606

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Promoter

Mouse Pgk1

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCyPet-Rac1(T17N)

Plasmid

#22784

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (T17N) (RAC1 Human, A. victoria)

Tags

CyPet

Expression

Mammalian

Mutation

Rac1 T17N.

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
J23100-mRFP1-331Bb

Plasmid

#78271

Purpose

mRFP1 behind constitutive promoter J23100 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23100-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-Rabin8

Plasmid

#118070

Purpose

Expresses Flag-tagged human Rabin8 in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

human Rabin8 (RAB3IP Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-MXD1

Plasmid

#178443

Purpose

doxycycline-inducible expression of Human MXD1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

MXD1 (MXD1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTet01

Plasmid

#78283

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Dppa2

Plasmid

#40799

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Dppa2 (Dppa2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pBabe-CyPet-Rac1

Plasmid

#24534

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (RAC1 Human)

Use

Retroviral

Tags

CyPet

Expression

Mammalian

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
attB53-Pac-TRE-mCherry-attB53

Plasmid

#183611

Purpose

RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

TRE-mCherry

Use

Rmce

Promoter

TRE

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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XE124 XDsh delta DEP-GFP-CS2+

Plasmid

#16785

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta-DEP (dvl2.L Frog)

Use

Xenopus expression

Tags

GFP

Mutation

contains Dsh amino acids 1-433 and 503-736 (delet…

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Hes5

Plasmid

#61536

Purpose

Expresses Hes5 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes5 (Hes5 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pMP71-mIL15-IL12rb1-sushi-il12rb2

Plasmid

#174606

Purpose

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183605

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Promoter

CAG

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-Puro

Plasmid

#44195

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Frb, Hu Frb x2(WT Frb+codon Wobble Frb), and V5/e…

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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CD200RCd4d3+4-bio

Plasmid

#32406

Purpose

A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 Receptor extracellular domain (Cd200r1 Rat)

Tags

Cd4d3+4 and biotinylation peptide

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-STAT2

Plasmid

#178449

Purpose

doxycycline-inducible expression of Human STAT2 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

STAT2 (STAT2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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CD200RCd4d3+4-blac

Plasmid

#32407

Purpose

A positive control receptor bait (rat CD4d3+4-blac) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 Receptor extracellular domain (Cd200r1 Rat)

Tags

Cd4d3+4 and beta-lactamase

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-SP110

Plasmid

#178448

Purpose

doxycycline-inducible expression of Human SP110 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

SP110 (SP110 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-SUB1

Plasmid

#178440

Purpose

doxycycline-inducible expression of Human SUB1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

SUB1 (SUB1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
XE123 XDsh delta PDZ-GFP- CS2+

Plasmid

#16786

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta-PDZ (dvl2.L Frog)

Use

Xenopus expression

Tags

gfp

Mutation

contains Dsh amino acids 1-267 and 381-736 (delet…

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only