We narrowed to 5,897 results for: ARL

1. pSD090A

   Plasmid

   #218132

   Purpose

   Hybrid circuit displaying mmCRBN bait and driving expression of luxAB

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   Crbn (Crbn Mouse)
   luxAB
   

   Use

   Tags

   434cI_RR69

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSD138A

   Plasmid

   #218130

   Purpose

   Hybrid circuit displaying hsCRBN bait (P22 anchor) and driving expression of luxAB

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   CRBN (CRBN Human)
   luxAB
   

   Use

   Tags

   scP22

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSD094B

   Plasmid

   #218131

   Purpose

   Hybrid circuit displaying mmCRBN bait and driving expression of gIII

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   Crbn (Crbn Mouse)
   M13 pIII
   

   Use

   Tags

   434cI_RR69

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ADRA2C-DuET

   Plasmid

   #213179

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADRA2C (ADRA2C Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. B2M-SDMutation-gRNA

   Plasmid

   #215547

   Purpose

   gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   B2M (B2M Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-PARP1–eGFP

   Plasmid

   #211552

   Purpose

   A piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. INS-3'gRNA

   Plasmid

   #210468

   Purpose

   gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   INS (INS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SpyTag003(M115G)-Talin rod-mCherry

   Plasmid

   #210026

   Purpose

   Expression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SpyTag003 M115G mutation to modify SpyCatcher003 …

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SpyTag003(M115A)-Talin rod-mCherry

   Plasmid

   #210025

   Purpose

   Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SpyTag003 M115A mutation to modify SpyCatcher003 …

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SpyTag003(V114T, V116T)-Talin rod-mCherry

    Plasmid

    #210027

    Purpose

    Expression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    SpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SpyTag003 V114T and V116T mutations to modify Spy…

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EGFP-Talin head-SpyCatcher003(K31TAG)

    Plasmid

    #210023

    Purpose

    Expression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    EGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SpyCatcher003 with K31TAG (Amber stop codon) muta…

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXPR_068

    Plasmid

    #164555

    Purpose

    Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

    Insert

    sgRNA cassette
    

    Use

    CRISPR and Lentiviral; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET19-TdTc253

    Plasmid

    #207464

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTc253 (Dntt Mouse)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Ser253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET19-TdTc188

    Plasmid

    #207463

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    TdTc188 (Dntt Mouse)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Cys216Ser, Cys302Ala, Cys378Ala, and Cys438Ser

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET19-TdTc180

    Plasmid

    #207462

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    TdTc180 (Dntt Mouse)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Glu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET19-TdTc302

    Plasmid

    #207461

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    TdTc302 (Dntt Mouse)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXPR_023d-sgCiPOLR2D

    Plasmid

    #202445

    Purpose

    Expression of a CRISPRi guide targeting POLR2D

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    POLR2D gRNA (POLR2D Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXPR_003-sgUBR4

    Plasmid

    #202442

    Purpose

    Expression of a guide RNA targeting UBR4

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    UBR4 gRNA (UBR4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXPR_003-sgUBA6

    Plasmid

    #202440

    Purpose

    Expression of a guide RNA targeting UBA6

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    UBA6 gRNA (UBA6 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSN554

    Plasmid

    #177361

    Purpose

    Expresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tag

    Depositor

    Shinsuke Niwa

    Article

    Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

    Insert

    KIF1A (KIF1A Human)
    

    Use

    Tags

    Leucine zipper, StrepII tag, and mScarlet

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge