We narrowed to 25,468 results for: FER

1. pCS2_Firefly_degron-60A

   Plasmid

   #200396

   Purpose

   A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   Firefly luciferase - degron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. eGFP Y93H AC editing reporter

   Plasmid

   #198883

   Purpose

   Express eGFP fluorescence after Y93H correction and mCherry as transfection control

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Y93H, Gly92(GGT-to-GGA)

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJOG1368

   Plasmid

   #167301

   Purpose

   Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Insert

   FCY-UPP fusion gene
   

   Use

   Synthetic Biology; Moclo level 0 vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Arf Q71L CFP

   Plasmid

   #128149

   Purpose

   Expression of Arf Q71L

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 2002 May 9;417(6885):187-93. doi

   Insert

   Arf1 (ARF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q71L

   Promoter

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. XNM-Luc

   Plasmid

   #35157

   Depositor

   Linda Penn

   Article

   Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

   Insert

   Myc promoter (MYC Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Contains 152 bp of the myc promoter

   Promoter

   c-myc

   Available since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pFRLuc-CoV2-WT

   Plasmid

   #177614

   Purpose

   Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKESaPar

   Plasmid

   #211829

   Purpose

   Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBSV2G_P0826-BB0323-mCherryBb

   Plasmid

   #118244

   Purpose

   Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   BB0323-mCherry translational fusion under the control of the bb0826 promoter
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET32a-hTGFa

   Plasmid

   #199236

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Transforming growth factor alpha, partial (TGFA Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEC726

    Plasmid

    #190814

    Purpose

    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    S. pyogenes dCas9 codon optimized for Streptomyce…

    Promoter

    SP30

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKERepPar

    Plasmid

    #211827

    Purpose

    Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP GLTP

    Plasmid

    #170746

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac_HT_B_6xHis-TEV-GFP-NBR1

    Plasmid

    #190928

    Purpose

    Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    NBR1 (NBR1 Human)
    

    Use

    Tags

    Strep-TEV-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmIL-6 mut NF-kB

    Plasmid

    #61293

    Purpose

    drives luciferase from mouse IL-6 promoter with mutation in NF-kB site

    Depositor

    Gail Bishop

    Article

    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

    Insert

    IL-6 promoter (Il6 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutated NF-kB binding site from GTGGGATTTTCCCA to…

    Promoter

    IL-6 promoter with mutant NF-kB binding site

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

15. EC2_3_dCas9_Mxi1_sgRNA

    Plasmid

    #163708

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    Mxi1+SV40 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115213

    Purpose

    Expresses RanCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    RanCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)/AUG-nLuc-3XFLAG

    Plasmid

    #127299

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTXB1-EXO1b

    Plasmid

    #68267

    Purpose

    Bacterial expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Use

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    T7

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1 3xFlag IFIT5

    Plasmid

    #53556

    Purpose

    mammalian expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

20. CapChR1_pmCherry-N1

    Plasmid

    #188031

    Purpose

    Expresses CapChR1-mCherry in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR1-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S63D, L132C, T159C and N258E mutations in CrChR2

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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