We narrowed to 10,056 results for: Uty

1. pBAD/HisD-PATagRFP

   Plasmid

   #31945

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   araB

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAD-CMV-STAT5A-CA-CMV-GFP

   Plasmid

   #83255

   Purpose

   Adenoviral expression of constitutively active STAT5A with co-expression of GFP

   Depositor

   Andrew Brooks

   Article

   Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

   Inserts

   Stat5a (Stat5a Mouse)
   eGFP
   

   Use

   Adenoviral

   Mutation

   constitutively active

   Promoter

   CMV

   Available Since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVSVG-PATagRFP

   Plasmid

   #31947

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Tags

   VSVG

   Expression

   Mammalian

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   CMV

   Available Since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. His_Naa80d in pTYB12

   Plasmid

   #188455

   Purpose

   Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   Naa80 (NAA80 Human)
   

   Tags

   intein-CBD, 6xHis

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSW149

   Plasmid

   #122248

   Purpose

   pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.

   Depositor

   Sarah Walker

   Article

   Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

   Insert

   TIF4631 (TIF4631 Budding Yeast)
   

   Tags

   Intein-Chitin-binding domain

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. BICDN-CIBN

   Plasmid

   #102253

   Purpose

   Expresses fusion of dynein adapter BICDN (1-594) and CIB1 (1-170)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   BICDN-CIB1 (CIB1 Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tet-CA-Src-GFP

   Plasmid

   #83469

   Purpose

   Lentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cells

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   Src (SRC Chicken)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Y527F (constitutively active)

   Promoter

   doxycycline-inducible promoter

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF Synaptophysin-GCamp6s

   Plasmid

   #188981

   Purpose

   Presynaptic fluorescent Ca2+ indicator

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Synaptophysin (Syp Synthetic, Rat)
   

   Expression

   Mammalian

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE8e-dimer

   Plasmid

   #138490

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-nSpCas9
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-hSyn-evoCjCas9-ABE8e_gRNA

    Plasmid

    #202561

    Purpose

    hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-ABE8e
    

    Use

    AAV

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PITPbeta-GFP

    Plasmid

    #58274

    Purpose

    mammalian expression of GFP-PITP beta

    Depositor

    Shamshad Cockcroft

    Article

    Morgan et al Biochem J. 2006 Sep 15;398(3):411-21.

    Insert

    PITP beta (PITPNB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCHC005 (∆RE)

    Plasmid

    #226199

    Purpose

    Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for type I restriction-modification system restriction subunit H16_A0006
    

    Use

    Synthetic Biology

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. EGFP-NIK

    Plasmid

    #111197

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

    Insert

    NIK (MAP3K14 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGI3EM22C

    Plasmid

    #135488

    Purpose

    Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr LifeAct-tdTomato
    

    Tags

    LifeAct

    Expression

    Bacterial and Yeast

    Promoter

    SpunH2A/Bpr (divergent)

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDR-GFPuniv

    Plasmid

    #46085

    Purpose

    In vivo recombination assay fluorescent reporter

    Depositor

    Raymond Monnat

    Article

    Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

    Insert

    DR-GFPuniv reporter
    

    Use

    Recombination reporter plasmid

    Expression

    Mammalian

    Mutation

    E5G and K163R mutations in EGFP relative to wild-…

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. SG3ΔENV RT D185A

    Plasmid

    #145782

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Reverse Transcriptase
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    Changed Reverse Transcriptase Aspartic Acid 185 t…

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSD103D

    Plasmid

    #218134

    Purpose

    For competition during selection with hybrid circuit. Expresses MBP fused to evolved degron SD55

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD55
    

    Tags

    MBP

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJM189A

    Plasmid

    #218135

    Purpose

    For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD0
    

    Tags

    rpoZ

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Tsapphire-StagRFP-SrcAR

    Plasmid

    #173018

    Purpose

    Long-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Tsapphire-StagRFP-SrcAR
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-SlugCBE-NNG-P2A-puro

    Plasmid

    #214355

    Purpose

    Expresses SlugCBE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCBE-NNG and puromycin resistance genes
    

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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