We narrowed to 14,086 results for: cas9

1. pDB4281

   Plasmid

   #98700

   Purpose

   A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. DS-NMcas

   Plasmid

   #48646

   Purpose

   Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   NM tracrRNA
   

   Use

   CRISPR

   Promoter

   NM tracrRNA promoter and proC

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330A-1x7

   Plasmid

   #58771

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAH237

   Plasmid

   #121146

   Purpose

   Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

   Depositor

   Katsunori Tanaka

   Article

   Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

   Inserts

   gRNA cassette
   humanized Streptococcus pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Flag

   Expression

   Yeast

   Promoter

   S.pombe rrk1 and nmt41

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMB66

   Plasmid

   #47946

   Purpose

   Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

   Expression

   Worm

   Promoter

   hsp-16.48

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-dSa-VP64

   Plasmid

   #99680

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VP64

   Mutation

   dead Cas9

   Available Since

   Oct. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. M-SPcas

   Plasmid

   #48668

   Purpose

   Mammalian S. pyogenes Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Promoter

   CMV

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. DS-ST1casN-

   Plasmid

   #48659

   Purpose

   Bacterial nuclease-null ST1 Cas9 expression

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9, nuclease-null
   ST1 tracrRNA
   

   Use

   CRISPR

   Mutation

   D to A, DH to AA, N to A

   Promoter

   proC

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. DS-ST1cas

   Plasmid

   #48647

   Purpose

   Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   ST1 tracrRNA
   

   Use

   CRISPR

   Promoter

   proC

   Available Since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMB67

    Plasmid

    #47947

    Purpose

    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and egl-13 NLS

    Expression

    Worm

    Promoter

    hsp-16.48

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. DS-TDcasN-

    Plasmid

    #48660

    Purpose

    Bacterial nuclease-null TD Cas9 expression

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Inserts

    Cas9, nuclease-null
    TD tracrRNA
    

    Use

    CRISPR

    Mutation

    D to A, DH to AA, N to A

    Promoter

    proC and tracrRNA promoter

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSHS293

    Plasmid

    #87348

    Purpose

    D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/D435C/C574S/E945C

    Promoter

    T7

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. BCJJ344

    Plasmid

    #117503

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtUBI10

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSHS248

    Plasmid

    #87354

    Purpose

    S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S/S867C/N1054C

    Promoter

    T7

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMLS544

    Plasmid

    #188882

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. dSp-VP64-RTA

    Plasmid

    #99675

    Purpose

    Expresses dSp Cas9 fused at C term. To VP64 and RTA activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    VP64-RTA

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMLS546

    Plasmid

    #188883

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Expression

    Worm

    Mutation

    C. elegans codom optimization

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. BCJJ340

    Plasmid

    #117502

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtUBI10

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cas-Acr (insertion 5)

    Plasmid

    #128116

    Purpose

    Expresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (insertion 5)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cas-Acr (N39A)

    Plasmid

    #128117

    Purpose

    Expresses SpyCas9 fused to attenuated AcrIIA4 (N39A)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (N39A)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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