We narrowed to 6,646 results for: Lta;

Showing: 3741 - 3760 of 6646 results

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pAAVS1-NDi-CRISPRi (Gen1)

Plasmid

#73497

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB-P2A-mCherry
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRi (Gen2)

Plasmid

#73498

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2-UAS-zmiRFP

Plasmid

#108415

Purpose

Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

zmiRFP

Use

Zebrafish

Promoter

UAS

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLCA.66/2272

Plasmid

#22733

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox66-pU(delta)TK-EM7Neo-Lox2272

Use

Cre/Lox; Bac recombineering

Mutation

This vector was designed for assembling gene targ…

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-miRFP

Plasmid

#108416

Purpose

Monomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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cacnb4 (rat) in pMT2 vector

Plasmid

#107426

Purpose

Expresses Calcium channel beta4 auxillary subunit in mammalian cells

Depositor

Annette Dolphin

Article

Brodbeck et al J Biol Chem. 2002 Mar 8;277(10):7684-93.

Insert

cacnb4 (Cacnb4 Rat)

Expression

Mammalian

Promoter

Ad MLP

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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tet-pLKO.neo_shGFP

Plasmid

#110470

Purpose

Control, silence GFP gene, doxycycline inducible, neomycin selection

Depositor

Sandra Martha Gomes Dias

Article

Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

Insert

GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO (RNA PolIII)

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 368STOP

Plasmid

#102596

Purpose

Lentivector to express STING V155M delta 368 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 368

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRFP-N1

Plasmid

#108409

Purpose

Monomeric near-infrared fluorescent protein miRFP in mammalian expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP

Plasmid

#108410

Purpose

Monomeric near-infrared fluorescent protein miRFP in bacterial expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Expression

Bacterial

Promoter

araB

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRFP-beta-Actin-C-19

Plasmid

#108412

Purpose

Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

beta-Actin

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

Plasmid

#102594

Purpose

Lentivector to express STING V155M delta 342 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 342

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

Plasmid

#102595

Purpose

Lentivector to express STING V155M delta 354 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 354

Promoter

SFFV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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shCCL20

Plasmid

#65096

Purpose

retroviral expression of CCL20 shRNA

Depositor

Pau Sancho-Bru

Article

Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

Insert

Ccl20 shRNA (Ccl20 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pBKWH-Lam

Plasmid

#133899

Purpose

Negative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

LMNA (Lam) (LMNA Human)

Expression

Yeast

Promoter

T7

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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CSNK1D gRNA (BRDN0001149348)

Plasmid

#76716

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1D

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1D (CSNK1D Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-d200

Plasmid

#114047

Purpose

Expression of exogenous LAP tagged MPS1 delta 200 (NTE and TPR are deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-d200 (TTK Human)

Expression

Mammalian

Mutation

delta 200 (deletion of NTE and TPR domains)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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DGKD gRNA (BRDN0001146827)

Plasmid

#77257

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKD

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKD (DGKD Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKD gRNA (BRDN0001147131)

Plasmid

#77258

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKD

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKD (DGKD Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKD gRNA (BRDN0001148084)

Plasmid

#77259

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKD

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKD (DGKD Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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