We narrowed to 10,128 results for: Met

Showing: 3741 - 3760 of 10128 results

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pXWJ109HgAmp30E

Plasmid

#123149

Purpose

Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Publication

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, PmerT, PhrpLE

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAH233

Plasmid

#121144

Purpose

Expression of gRNA with BbsI placeholder for target cloning

Depositor

Katsunori Tanaka

Publication

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Insert

gRNA cassette

Use

CRISPR

Expression

Yeast

Promoter

S.pombe rrk1

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shSHMT2

Plasmid

#106319

Purpose

Express shRNA targeting SHMT2

Depositor

Richard Possemato

Publication

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

shRNA targeting SHMT2

Use

Lentiviral and RNAi

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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MXS_P2A

Plasmid

#62433

Purpose

MXS_chaining vector with P2A

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Self-cleaving peptide

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_bGpA

Plasmid

#62424

Purpose

MXS_chaining vector with bGpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

beta-Globin poly A

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pYes2-TaPCS1

Plasmid

#49767

Purpose

Expresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Publication

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

TaPCS1 (PCS1 Triticum aestivum)

Expression

Yeast

Promoter

GAL1

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MMACHC

Plasmid

#39096

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MMACHC (MMACHC Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pX458_GAPDH-sgRNA

Plasmid

#249575

Purpose

Expresses SpCas9, GFP, and sgRNA targeting GAPDH.

Depositor

Joe Zhang

Publication

Zhang et al STAR Protoc. 2025 Jul 4;6(3):103928. doi

Insert

GAPDH-sgRNA

Use

CRISPR

Promoter

U6 promoter

Available since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pET28a-AncMEcoGII250

Plasmid

#205025

Purpose

Expressing engineered M.EcoGII with higher thermostability

Depositor

Paola Laurino

Publication

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

Ancestral M.EcoGII 250

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Dimer cleaved

Plasmid

#198199

Purpose

FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

Depositor

Christian Wahl-Schott

Publication

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mVenus-T2A-mTurquoise2

Expression

Mammalian

Mutation

A206K both

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Mito-meCpG

Plasmid

#186462

Purpose

Expresses GFP tagged methyl-CpG recognition motif targeted to mitochondria in mammalian cells.

Depositor

Chongli Yuan

Publication

Zhao et al ACS Sens. 2021 Oct 22;6(10):3575-3586. d

Insert

dimeric MBD1 (MBD1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLVU-coHPDL WT

Plasmid

#174128

Purpose

Expresses wild type codon-optimized HPDL in mammalian cells

Depositor

Michael Pacold

Publication

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Codon optimized

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE046 ZF43x12-C mKate2 (TUPV1)

Plasmid

#138726

Purpose

mMoClo TUPV, with ZF43x12-C promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Compact

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET30a-SRAP-delCys2

Plasmid

#84568

Purpose

Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

Depositor

Douglas Feldman

Publication

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Srap1 (Hmces Mouse)

Tags

6xHis and S-Tag

Expression

Bacterial

Mutation

deletion of Cys2

Promoter

T7

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pERB160

Plasmid

#58285

Purpose

const. Mis12-FKBP3; ind. FRB-mCH-Mad1

Depositor

Michael Lampson

Publication

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA

Tags

mCherry

Expression

Mammalian

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MXS_H2B

Plasmid

#62432

Purpose

MXS_chaining vector with H2B

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Histone 2B with leading Kozak sequence

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pERB111

Plasmid

#58281

Purpose

constitutive miRempty_Mis12-GFP-FKBP3; inducible mCh-FRB

Depositor

Michael Lampson

Publication

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

empty miRNA
Mis12-GFP-FKBPx3
inducible mCh-FRB

Tags

GFP and mCherry

Expression

Mammalian

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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tdEos-Vimentin-7

Plasmid

#57688

Purpose

Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Vimentin (VIM Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-TAL-NC2

Plasmid

#43856

Depositor

Takashi Yamamoto

Publication

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Promoter

CAG

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-DJ1-M26I

Plasmid

#29353

Depositor

Mark Cookson

Publication

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Met26Ile

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only