We narrowed to 3,867 results for: mak

1. pLX304-BCL-XL-499/500R

   Plasmid

   #203575

   Purpose

   Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   silent point mutations to make resistant shRNA 49…

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD649-HAsp-SEMA7A-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156922

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA7A (SEMA7A Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mTet1 flox donor

   Plasmid

   #117147

   Purpose

   Making Tet1 conditional knock out

   Depositor

   Jacob Hanna

   Article

   Zviran et al ssrn.3155731

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   Mouse Targeting

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mTet3 flox donor

   Plasmid

   #117146

   Purpose

   Making Tet3 conditional knock out

   Depositor

   Jacob Hanna

   Article

   Zviran et al ssrn.3155731

   Insert

   Tet3 (Tet3 Mouse)
   

   Use

   Mouse Targeting

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Strain KJ1C

   Bacterial Strain

   #21874

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Protoc. 2009. 4(10):1471-501.

   Bacterial Resistance

   Tetracycline

   Available Since

   Nov. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

6. A3Bi-ctd E255A -Cas9n-UGI-NLS

   Plasmid

   #109429

   Purpose

   Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Insertion of an L1 intron into the coding sequenc…

   Promoter

   CMV

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB OROV Gc Spike H6

   Plasmid

   #229662

   Purpose

   Make PiggyBac stable cell line expressing Oropouche virus BeAn 19991 Gc spike region (residues 482–894 of M polyprotein) with C-terminal His6 tag

   Depositor

   Stephen Graham

   Article

   Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

   Insert

   OROV Gc spike
   

   Use

   Piggybac

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   contains residues 482-894 only

   Promoter

   TRE

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-GST-ATG9 (828-839)-ATG101 (1-198)

   Plasmid

   #188073

   Purpose

   To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 and ATG101 (ATG9A, ATG101 Human)
   

   Tags

   GST tag

   Expression

   Mammalian

   Mutation

   codon-optimized ATG9 (828-839) and codon-optimize…

   Promoter

   CMV

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHD-dsRed-attP_dCrk_flankingHRs

   Plasmid

   #131141

   Purpose

   Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Inserts

   Crk Upstream Homology Region (Crk Fly)
   Crk Downstream Homology Region (Crk Fly)
   

   Use

   CRISPR and Cre/Lox; Homology directed repair temp…

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi

    Plasmid

    #216166

    Purpose

    Express the gRNAs for the human FOXA1/A2/A3-CRISPRi

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA-FOXA1-Rank1, sgRNA-FOXA2-Rank2, sgRNA-FOXA2-Rank1, and sgRNA-FOXA3-Rank1 (FOXA1, FOXA2, FOXA3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    phU6, ph7SK, phH1, pmU6

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Strain CSH100

    Bacterial Strain

    #21875

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Protoc. 2009. 4(10):1471-501.

    Bacterial Resistance

    None

    Available Since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

12. HL 3325 (=HL 770 + rhyB knockout_KanR)

    Bacterial Strain

    #30065

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Bacterial Resistance

    Kanamycin

    Species

    Escherichia coli

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

13. BL21 ΔrecBCD

    Bacterial Strain

    #176581

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Chase Beisel, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

14. BL21 Rosetta2 ΔrecBCD

    Bacterial Strain

    #176583

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

15. BL21 ΔrecB

    Bacterial Strain

    #176580

    Purpose

    E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Chase Beisel, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

16. BL21 Rosetta2 ΔrecB

    Bacterial Strain

    #176582

    Purpose

    E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

17. BL21 Rosetta2 ΔrecBCD GamS

    Bacterial Strain

    #176586

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

18. BL21 Rosetta2 GamS

    Bacterial Strain

    #176584

    Purpose

    E. coli BL21 strain for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

19. BL21 Rosetta2 ΔrecB GamS

    Bacterial Strain

    #176585

    Purpose

    E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

20. EH1

    Bacterial Strain

    #102804

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted.

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only