We narrowed to 16,618 results for: puromycin

1. pCSIIpuro2-H2B-iRFP

   Plasmid

   #197352

   Purpose

   Encoding nuclear-targeted iRFP713.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   iRFP with nucleus-targeting sequence
   

   Use

   Lentiviral

   Tags

   H2B (human histone 2B)

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lox-Stop-Lox TOPO

   Plasmid

   #11584

   Purpose

   Cre-dependent activation of gene of interest by removing Stop sequences; Puro selection.

   Depositor

   Tyler Jacks

   Article

   Jackson et al Genes Dev. 2001 Dec 15. 15(24):3243-8.

   Insert

   loxP-STOP-loxP
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPCX mito roGFP2-Orp1

   Plasmid

   #64992

   Purpose

   Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   mitochondrial targeting sequence

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-HA-COPS5

   Plasmid

   #22541

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   COPS5 (COPS5 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. mATF4

   Plasmid

   #24874

   Depositor

   Fumihiko Urano

   Article

   Ghosh et al PLoS One. 2010 . 5(3):e9575.

   Insert

   mouse ATF4 (Atf4 Mouse)
   

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   May 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pX459-HypaCas9-AAVS1_sgRNA

   Plasmid

   #183891

   Purpose

   pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 sgRNA spacer (AAVS1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. KA2058_pX330A-G2P

   Plasmid

   #124202

   Purpose

   Expression of SpCas9 and sgRNA

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLPCX-PECAMTS

   Plasmid

   #45850

   Depositor

   Martin Schwartz

   Article

   Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

   Insert

   PECAM1 (PECAM1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. mc2 puro

   Plasmid

   #206215

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-puro-EGFP-FAK

    Plasmid

    #38194

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    PTK2 protein tyrosine kinase 2 (Ptk2 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-entry-puro

    Plasmid

    #85745

    Purpose

    Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBbi_RP_lbMicroID2-HA

    Plasmid

    #188522

    Purpose

    Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    lbMicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PresentER-SIINFEKL (GFP)

    Plasmid

    #102944

    Purpose

    PresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Insert

    SIINFEKL (OVAL Synthetic, Chicken)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    OVA257-264

    Promoter

    LTR

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-Pur-CAG-mCherry

    Plasmid

    #80946

    Purpose

    human AAVS1 site targeting donor plasmid for knocking-in mCherry expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLPCX-PECAMTL

    Plasmid

    #45851

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    PECAM1 (PECAM1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEFL-EGFP-TEADiv2

    Plasmid

    #228872

    Purpose

    Expression of optimized TEAD inhibitor (TEADiv2): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

    Insert

    TEADiv2
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-opto-hMLKL

    Plasmid

    #208789

    Purpose

    Inducibly expresses human MLKL, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    MLKL (MLKL Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-AAVS1-PGK-Puro

    Plasmid

    #227269

    Purpose

    Empty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC026

    Plasmid

    #227598

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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