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EF1a_ESRRG_P2A_Hygro_Barcode

Plasmid

#120435

Purpose

Barcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ESRRG (ESRRG Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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nos-Cas9.874Z1

Plasmid

#112685

Purpose

Express hSpCas9 under nanos promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

nanos

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMonAID_dCas9-PmCDA_Hyg_ALS

Plasmid

#91692

Purpose

Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A and H840A for dead Cas9

Promoter

2x35S

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pKD233.7-3

Plasmid

#90955

Purpose

Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Tetrathionate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23114, PltetO-1, and PttrB185-269

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB-gDA3m

Plasmid

#208707

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons in a cre-dependent manner

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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SpdCas9 KRAB

Plasmid

#188499

Purpose

Expresses FLAG tagged SpdCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT001

Plasmid

#182711

Purpose

aTc inducible dCasRx

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

2x-FLAG

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CAT

Plasmid

#173943

Purpose

Bacterial expression of isolated PARP1 CAT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CAT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Active

Plasmid

#173072

Purpose

Active SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pKL09-GCaMP6f-mScarlet bb118

Plasmid

#158979

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-mScarlet fusion

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-6His-SUMO-Nsp10

Plasmid

#154959

Purpose

Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

Depositor

Justin Kollman

Insert

6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)

Tags

6His-Thrombin-SUMO

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pNCST-avGFP

Plasmid

#129508

Purpose

Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

avGFP

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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NYESO alpha into TRAC HDRT Source (pTR 223)

Plasmid

#112023

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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Ubiq-Cas9.874W

Plasmid

#112686

Purpose

Express hSpCas9 under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

Ubiquitin-63E promoter

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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CLTA-GFP HDRT Source (pTR 153)

Plasmid

#112016

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-GFP HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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tCRMod-GGSGGS5

Plasmid

#111760

Purpose

Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-GGSGGS5-mRuby2 fusion

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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GW1-mito-roGFP1-mApple

Plasmid

#107218

Purpose

Mammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.

Depositor

Mathew Tantama

Article

Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

Insert

roGFP1-mApple

Tags

4xCox8

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B2

Plasmid

#103003

Purpose

non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B2 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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SaBE3-Gam

Plasmid

#100810

Purpose

Expresses SaBE3-Gam in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

SaBE3-Gam

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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tdTomato-H2B-C-10

Plasmid

#58102

Purpose

Localization: Nucleus/Histones, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

tdTomato

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only