167,729 results

1. HA-hULK1

   Plasmid

   #31963

   Depositor

   Do-Hyung Kim

   Article

   Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

   Insert

   ULK1 (ULK1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Sept. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nsp3 -EGFP

   Plasmid

   #165108

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp3 (ORF1ab SARS-COV-2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

   Plasmid

   #223249

   Purpose

   Mammalian expression of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCardinal-N1

   Plasmid

   #54590

   Purpose

   Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Type

   Empty backbone

   Tags

   mCardinal

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pPGK-T7/2-CD44v6 (fl)

   Plasmid

   #137816

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6 (fl) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R296K in the V6 region [Please see depositor comm…

   Promoter

   PGK

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-(-)-Pur

   Plasmid

   #200458

   Purpose

   Eukaryotic expression vector

   Depositor

   Bertrand Collet

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pscAAV-GFP

   Plasmid

   #32396

   Depositor

   John T Gray

   Article

   Gray et al Methods Mol Biol. 2011;807:25-46.

   Insert

   GFP
   

   Use

   AAV; Adeno-associated virus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV5)

   Viral Prep

   #162379-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

9. PLKO.1-Scrambled

   Plasmid

   #136035

   Purpose

   Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   None (Scrambled)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-mitoLbNOX

    Plasmid

    #74448

    Purpose

    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

    Insert

    mitoLbNOX
    

    Tags

    Flag tag with linker and mitochondrial targeting …

    Mutation

    Human codon optimized

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV9)

    Viral Prep

    #100845-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

12. TRUPATH Triple GaoB

    Plasmid

    #196052

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-FLAG-mCherry-C1-centrin-1

    Plasmid

    #73333

    Purpose

    Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

    Depositor

    Manuel Thery

    Insert

    centrin-1 (CETN1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

    Plasmid

    #125712

    Purpose

    Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    eOPN3
    

    Use

    AAV

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 331-429

    Promoter

    short version of αCamKinase II promoter (0.4kb)

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-EF1Alpha-Tet-on 3G

    Plasmid

    #118592

    Purpose

    Expresses Tet-on 3G from the EF1Alpha promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Tet-on 3G
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Human Epigenetic Knockout Library

    Pooled Library

    #162256

    Purpose

    Lentiviral CRISPR library targets human epigenetic genes

    Depositor

    Kivanc Birsoy

    Article

    Williams et al Cell Metab. 2020 Apr 7;31(4):852-861.e6.

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

17. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

    Plasmid

    #193356

    Purpose

    VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CMV.ffLuciferase.SV40 (AAV8)

    Viral Prep

    #105532-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV.CMV.ffLuciferase.SV40 (#105532). In addition to the viral particles, you will also receive purified pAAV.CMV.ffLuciferase.SV40 plasmid DNA. CMV-driven ffLuciferase control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CMV

    Tags

    ffLuciferase

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

19. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid

    #220800

    Purpose

    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

    Depositor

    Elizabeth Kirby

    Article

    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

    Insert

    Mars1 (Mars1 Mouse)
    

    Use

    Lentiviral

    Tags

    2xFLAG and T2A-mCherry

    Mutation

    mutation in MetRS to change aa 274 from L to G

    Promoter

    EF1a

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. CAG-Blimp1

    Plasmid

    #73995

    Purpose

    Plasmid expressing mouse Blimp1

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Blimp1 gene (Prdm1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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