We narrowed to 11,601 results for: 112

1. pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag

   Plasmid

   #185682

   Purpose

   Low-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Synthetic, Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   K599A

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PMmEos3.2

   Plasmid

   #203755

   Purpose

   Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   Lyn n-term (LYN Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-CMV-Grid2(I677C)

   Plasmid

   #164782

   Purpose

   cysteine mutated glutamate delta2 receptor (I677C) for the attachment of a photoswitchable tethered ligand

   Depositor

   Alexandre Mourot

   Article

   Lemoine et al Elife. 2020 Oct 28;9. pii: 59026. doi: 1

   Insert

   Grid2 I677C (Grid2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I677C

   Promoter

   CMV

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LentiGuide-Puro-NCOA7g1 (BB22)

   Plasmid

   #139457

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LentiGuide-Puro-NCOA7g2 (BB23)

   Plasmid

   #139458

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-ALK2 K235R

   Plasmid

   #80872

   Purpose

   mammalian expression of ALK2 K235R

   Depositor

   Aristidis Moustakas

   Article

   Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

   Insert

   ALK2 (ACVR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K235R

   Promoter

   CMV

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pDONR221_SLC45A4

   Plasmid

   #132179

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC45A4 (SLC45A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pHH0103_LYN-1/1

   Plasmid

   #91536

   Purpose

   Protein expression and purification of human SH3 domain construct LYN-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   LYN-1/1 (LYN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FLAG-KLF12/pcDNA3.1-Neo

   Plasmid

   #81069

   Purpose

   Expression of epitope-tagged human KLF12 in mammalian cells.

   Depositor

   Julian Downward

   Article

   Godin-Heymann et al Oncogene. 2016 Jun 23;35(25):3324-34. do

   Insert

   FLAG-KLF12 (KLF12 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag

    Plasmid

    #185681

    Purpose

    Low-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Deletion of lumenal domain

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FXYD2-luc

    Plasmid

    #31056

    Depositor

    Gerhart Ryffel

    Article

    Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

    Insert

    FXYD2 (FXYD2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    FXYD2 promoter from -2382 to -1

    Promoter

    Available since

    Aug. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Flrt1-Fc-His

    Plasmid

    #72073

    Purpose

    Expresses the extracellular region of the FLRT1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Flrt1 (Flrt1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag

    Plasmid

    #185684

    Purpose

    Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    K121Y

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX Lyn-SH2

    Plasmid

    #46452

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Lyn (LYN Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 106-213

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant

    Plasmid

    #149507

    Purpose

    Transient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Transient expression

    Tags

    TEV-3XHA-3XFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized Sequence, p.LL256-257AA

    Promoter

    CMV

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GPR45-Tango

    Plasmid

    #66360

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR45 (GPR45 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pPW3763 : CMVd3-HsIRE1dLKR-HaloTag

    Plasmid

    #185685

    Purpose

    Low-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Deletion of linker, kinase, and Rnase domains

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMX-IY LC3A

    Plasmid

    #89290

    Purpose

    For expression of the mammalian Atg8 protein LC3A without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    LC3A (MAP1LC3A Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. K15-TK/pGL3

    Plasmid

    #44267

    Depositor

    George Cotsarelis

    Article

    Ito et al Nat Med. 2005 Dec;11(12):1351-4. Epub 20

    Insert

    K15 promoter (-4.8) (Krt15 Mouse)
    

    Use

    Mouse Targeting; Thymidine kinase

    Tags

    HSV-1 TK

    Expression

    Mammalian

    Mutation

    Contains the murine K15 promoter (-4.8) fragment

    Promoter

    murine K15 promoter (-4.8) fragment

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pGL3-IRES-Lin28b-P3

    Plasmid

    #64794

    Purpose

    Luciferase expression driven by Lin28b promoter P3

    Depositor

    Joshua Mendell

    Article

    Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Lin28b promoter P3 (LIN28B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge