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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,138 results for: eng

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Showing: 3761 - 3780 of 14138 results

  3. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid
    #181792
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181792-plasmid-map-sequence-id-358226

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  9. 857 pBabe puroL PTEN G129R

    Plasmid
    #10772
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    G129R
    Available Since
    Sept. 27, 2005
    Availability
    Academic Institutions and Nonprofits only

  10. BARBEKO sgRNA library

    Pooled Library
    #174163
    Purpose
    Cytosine base editors for genome-scale knockout screens in human cells. Targets gene start codons, splice sites, or introduces premature termination codons.
    Depositor
    Wensheng Wei
    Article
    Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available Since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only

  12. gNS2B47NS3 S135A

    Plasmid
    #155317
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    S135A
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155317-plasmid-map-sequence-id-311234

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  15. ttAtCas12a+int

    Plasmid
    #182384
    Purpose
    Gives very high editing efficiency in barley. Also works in Brassica oleracea
    Depositor
    Wendy Harwood
    Article
    Lawrenson et al bioRxiv 2022.04.28.489853
    Insert
    LbCas12a coding sequence with D156R and Arabidopsis introns
    Use
    CRISPR
    Mutation
    D156R + 8 Arabidopsis introns added
    Promoter
    None
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182384-plasmid-map-sequence-id-361472

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  16. pLV hMyoD-IRES-dsRed

    Plasmid
    #66631
    Purpose
    Co-expresses human MyoD and dsRed
    Depositor
    Charles Gersbach
    Insert
    human MyoD IRES dsRed (MYOD1 Human)
    Use
    Lentiviral
    Promoter
    hUbc
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66631-plasmid-map-sequence-id-114011

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Showing: 3761 - 3780 of 14138 results