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single polypeptide FPX biosensor for both caspase-3 and -8

Plasmid

#60886

Purpose

FPX biosensor

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for caspase-3 and 8

Use

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
Tol2-LyzC-GFP-U6ac-rac2-guides

Plasmid

#168241

Purpose

"neutrophil specific GFP with ubiquitous rac2 sgRNAs"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

rac2 sgRNAs

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

Plasmid

#157984

Purpose

Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

Depositor

Lijia Ma

Article

Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

Insert

S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-U6ac-cdk2-guides

Plasmid

#168250

Purpose

"neutrophil specific GFP with ubiquitous cdk2 sgRNAs"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

cdk2 sgRNAs

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-LyzC-RacFRET-U6ac-rac2 guides

Plasmid

#168248

Purpose

"label active Rac under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

RacFRET

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCF821-sgCIDE-10_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211670

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-10 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-5_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211665

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-5 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-6_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211666

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-6 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-7_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211667

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-7 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-4_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211664

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-4 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-21_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211671

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-21 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-22_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211672

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-22 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211662

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-2 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-8_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211668

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-8 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-9_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211669

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-9 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-42_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211682

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-42 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-43_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211683

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-43 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF820-sgCIDE-1_U6-sgRNA-EF1a-mCherry2

Plasmid

#211649

Purpose

U6-sgRNA-EF1a-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

sgCIDE-1 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF820-sgCIDE-2_U6-sgRNA-EF1a-mCherry2

Plasmid

#211650

Purpose

U6-sgRNA-EF1a-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

sgCIDE-2 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-35_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211679

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-35 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge