We narrowed to 4,785 results for: pcr

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pCR3-FLAG-Ubc13-WT

Plasmid

#43938

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

ubiquitin-conjugating enzyme E2N (UBE2N Human)

Tags

2x FLAG

Expression

Mammalian

Available Since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Nat

Plasmid

#232097

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-PermE

Plasmid

#209446

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Promoter

PtipA; sgRNA expression from ermE* promoter

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCR-Flag-IKKbeta

Plasmid

#15465

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta (Ikbkb Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
pCry2PHR-mCh-RhoA

Plasmid

#42958

Purpose

Expresses optogenetically controlled RhoA

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xP3-RFP

Plasmid

#130276

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

3xP3-RFP

Use

CRISPR

Expression

Insect

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Gephyrin P1

Plasmid

#68816

Purpose

Flag tagged Geph mammalian expression

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

3xFLAG

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyrin…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pCRISPaint-3xP3-GFP

Plasmid

#130277

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

3xP3-GFP

Use

CRISPR

Expression

Insect

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xflag-PuroR

Plasmid

#80973

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

3xflag tag

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-TBX20a

Plasmid

#98620

Purpose

Gateway entry TOPO TA cloning vector for TBX20a

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

Tbx20a (Tbx20 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII-BbsI-sgRNAscaffold

Plasmid

#159352

Purpose

Expression vector for a customizable guide RNA

Depositor

Pim Pijnappel

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCR2.1 GFR alpha 2

Plasmid

#119899

Purpose

Mouse GFR alpha 2 In situ hybridization probe

Depositor

David Ginty, Wenqin Luo

Article

Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

Insert

GDNF family receptor alpha-2 (Gfra2 Mouse)

Use

In situ hybridization probe

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO BMI

Plasmid

#69796

Purpose

Gateway entry vector for BMI

Depositor

Gerard Cagney

Insert

BMI (BMI1 Human)

Expression

Bacterial

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only
pCR8/GW/TOPO PCGF2

Plasmid

#69791

Purpose

Gateway entry vector for PCGF2

Depositor

Gerard Cagney

Insert

PCGF2 (PCGF2 Human)

Expression

Bacterial

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only
pCRISPEY-GAL-Kan

Plasmid

#232099

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Hyg

Plasmid

#232098

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-1.1-1.10a

Plasmid

#69589

Purpose

OT-II TCR a chain cDNA in pCRII

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Insert

TCR alpha clone 1.1 (Tcra Mouse)

Expression

Bacterial

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only
pCry2PHR-mCh-Rac1

Plasmid

#42954

Purpose

Expresses optogenetically controlled Rac1

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-Rac1 (RAC1 Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCR2.1-KRAS-G12V

Plasmid

#129091

Purpose

KI KRAS G12V mutation into human KRAS locus

Depositor

Toshiro Sato

Article

Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

Insert

KRAS(G12V) (KRAS Human)

Use

Homologous recombination donor plasmid

Mutation

G12V

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR Human CASP9 -2

Plasmid

#198420

Purpose

Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Human CASP9 Specific gRNA (CASP9 Human)

Use

CRISPR

Tags

Cas9/Orange Fluorescent Protein Reporter

Expression

Mammalian

Promoter

U6; CMV

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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